|Application ||WB, IHC-P, E|
|Calculated MW||46169 Da|
|Purification||Antiserum to human RAD52 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.|
|Immunogen||Synthetic peptide corresponding to amino acids 360 - 375 of the Human RAD52 protein.|
|Shelf Life||18 months from date of despatch.|
|Other Names||DNA repair protein RAD52 homolog, RAD52|
|Target/Specificity||ABD11038 recognises Human Rad52, a protein which functions in DNA repair through the homologous recombination of DNA double strand breaks (DSBs). DSBs can be generated through exposure to ionizing radiation or by endogenous cellular reactions, and Rad52 acts during the latter stages of recombination after the DSBs are processed into single stranded DNA (ssDNA). Rad52 functions together with Rad51 and RPA (Replication Protein A), where it has two distinct activities. First, Rad52 and RPA form a co-complex on ssDNA, where Rad52 functions to recruit Rad51. In addition, Rad52 has ssDNA binding and annealing activities, particularly ssDNA saturated with RPA. Rad52 is present at low levels during the G1 phase of the cell cycle, when recombinatorial repair is absent. The level rises steadily through S phase reaching a maximum in G2 phase when homologous repair is also at its highest level.|
|Preservative & Stabilisers||0.01% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human RAD52 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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