|Application ||WB, IHC-P, E|
|Calculated MW||169863 Da|
|Purification||Antiserum to human GLI3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.|
|Immunogen||Synthetic peptide corresponding to amino acid sequence 41-57 of human Gli3 protein.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Transcriptional activator GLI3, GLI3 form of 190 kDa, GLI3-190, GLI3 full length protein, GLI3FL, Transcriptional repressor GLI3R, GLI3 C-terminally truncated form, GLI3 form of 83 kDa, GLI3-83, GLI3|
|Target/Specificity||ABD11045 recognizes the Human Zinc Finger protein 3 (GLI-3), also known as GLI-Kruppel family member 3, a 169.8kDa protein belonging to the GLI C2H2-type zinc finger protein of transcription factors.GLI-3 and the related GLI-2 proteins are the primary transcription factors that mediate Sonic hedgehog (Shh) signals in the mouse, GLI-2 being primarily a transcriptional activator while GLI-3 is a transcriptional repressor. GLI-3 plays a role in the development of the limbs and brain. It is expressed in a wide range of adult tissues including the colon, lung, spleen, testis and placenta.Defects in GLI3 are causative of a range of disease states including Pallister-Hall syndrome, type IV preaxial polydactyly, acrocallosal syndrome (ACS) and Greig cephalo-poly-syndactyly syndrome.GLI proteins are also implicated as potent oncogenes in the embryonal carcinoma cell.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-GLI3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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