|Application ||WB, IHC-P|
|Purification||Antiserum to human BRCC36 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.|
|Immunogen||An 18 amino acid peptide from near the amino terminus of human BRCC36.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||AHP1465 recognises human BRCA1/BRCA2 complex subunit 36 (BRCC36), a 36.7kDa nuclear protein. BRCC36, together with BRCA1, BRCA2, Rad51 and BRCC45, forms a holoenzyme complex that possesses an ubiquitin E3 ligase activity. Studies have demonstrated that BRCC36 functions in enhancing cellular survival following DNA damage and that it is essential for ionizing radiation induced BRCA1 phosphorylation and nuclear foci formation. Research has shown that BRCC36 is aberrantly expressed in the majority of breast tumours indicating a potential role in the pathogenesis of the disease. It has been hypothesized that BRCC36 could be a potentially important target in the treatment of radiation-resistant breast tumours.|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-BRCC36 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Dong, Y. et al. (2003) Regulation of BRCC, a holoenzyme complex containing BRCA1 and BRCA2, by a signalosome-like subunit and its role in DNA repair.Mol. Cell 12:1087-99.2. Chen, X. et al. (2006) BRCC36 is essential for ionizing radiation-induced BRCA1 phosphorylation and nuclear foci formation. Cancer Res. 66:5039-46.
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