|Application ||WB, IHC-P|
|Calculated MW||32668 Da|
|Purification||Antiserum to human ORAI1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.|
|Immunogen||A 16 amino acid peptide from near the carboxy terminus of human ORAI1.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Calcium release-activated calcium channel protein 1, Protein orai-1, Transmembrane protein 142A, ORAI1, CRACM1, TMEM142A|
|Target/Specificity||ABD11059 recognises human ORAI1, also known as Transmembrane protein 142A (TMEM142A), a 32.7 kDa multi-pass membrane protein belonging to the ORAI family. Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. ORAI1 is a four- transmembrane spanning protein that is an essential component of CRAC. ORAI1 functions as a highly selective Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+sensor.Defective ORAI1 in humans is the cause of one form of hereditary severe combined immune deficiency (SCID), which results in ablated T-cell Ca2+entry.ABD11059 is not expected to cross react with either ORAI2 or ORAI3.|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-ORAI1 (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Feske, S., et al. (2006) A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function.Nature; 441:179-852. Soboloff, J., et al. (2006) Calcium signals mediated by STIM and Orai proteins – a new paradigm in inter-organelle communication.Biochim. Biophys Acta.: 1763:1161-11683. Lewis, R.S. (2001) Calcium signaling mechanisms in T lymphocytes.Annu. Rev. Immunol.; 19:497-521
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