|Application ||WB, IHC-F|
|Calculated MW||31499 Da|
|Purification||Antiserum to human ORAI3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.|
|Immunogen||A 15 amino acid peptide from near the amino terminus of human ORAI3.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Protein orai-3, Transmembrane protein 142C, ORAI3, TMEM142C|
|Target/Specificity||ABD11060 recognises human ORAI3, also known as Transmembrane protein 142C (TMEM142C), a 31.5 kDa multi-pass membrane protein belonging to the ORAI family. Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. ORAI3 is one of two mammalian homologs of ORAI1, a four- transmembrane spanning protein that is an essential component of CRAC channels. ORAI3, along with ORAI1 and ORAI2, functions as a Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor. Studies indicate that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. ABD11060 is not expected to cross react with either ORAI1 or ORAI2.|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-ORAI3 (N-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Feske, S., et al. (2006) A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function.Nature; 441:179-852. Soboloff, J., et al. (2006) Calcium signals mediated by STIM and Orai proteins – a new paradigm in inter-organelle communication.Biochim. Biophys.Acta: 1763:1161-11683. Mercer, J.C., et al. (2006) Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1. J. Biol. Chem.; 281:24979-904. Lewis, R.S. (2001) Calcium signaling mechanisms in T lymphocytes.Annu. Rev. Immunol.; 19:497-521
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