|Application ||WB, IHC-P, IP|
|Calculated MW||101896 Da|
|Purification||Antiserum to human MCM2 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.|
|Immunogen||Synthetic peptide corresponding to an epitope within amino acid residues 1-50 of human MCM2 (NP_0045117.2).|
|Shelf Life||18 months from date of despatch.|
|Other Names||DNA replication licensing factor MCM2, 220.127.116.11, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, MCM2, BM28, CCNL1, CDCL1, KIAA0030|
|Target/Specificity||ABD11062 specifically recognises the nuclear protein MCM2 (mini chromosome maintenance protein 2), a putative DNA replicative helicase and member of the MCM family, critical during the initiation of DNA replication.During the G1 phase of the cell cycle the MCM proteins, part of the pre-replicative complex which assembles at replication origins, undergo phosphorylation by kinases including cyclin dependent kinase (CDK) and Cdc7/Dbf4 (DDK). Studies have shown that the phosphorylation of MCM2 occurs on multiple sites, namely Ser27, Ser41, Ser53, Ser108 and Ser139 and that phosphorylation results in a conformational change of the complex and activation of the helicase activity.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-MCM2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Tsuji, T. et al. (2006) Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells. Mol. Biol. Cell 17: 4459-4472.1. Forsburg, S.L. (2004) Eukaryotic MCM proteins: Beyond replication initiation. Microbiol. Mol. Biol. Rev. 68: 109-131.
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