|Calculated MW||77983 Da|
|Purification||Antisera to phosphorylated 5-lipoxygenase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||Synthetic phosphopeptide corresponding to an amino acid sequence within human 5-lipoxygenase which includes phosphorylated Ser523.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Arachidonate 5-lipoxygenase, 5-LO, 5-lipoxygenase, 220.127.116.11, ALOX5, LOG5|
|Target/Specificity||ABD11070 specifically recognises human 5-lipoxygenase (5-LO, also known as arachidonate 5-lipoxygenase) when phosphorylated at Ser523. 5-LO is an enzyme that catalyses the synthesis of leukotrienes (LT’s) from arachidonic acid (a polyunsaturated fatty acid), by the insertion of an oxygen into the arachidonic backbone. 5-LO requires calcium, ATP and the 5-LO-activating protein as cofactors for full activity. Leukotrienes are synthesised and secreted by leukocytes in response to immunologic or inflammatory stimuli, such as in asthma and allergic reactions. The biological effects of LT’s include leukocyte chemotaxis, pulmonary airway constriction, mucous hypersecretion, vasoconstriction and increased vascular permeability. I recent years 5-LO has also been implicated in the regulation of cell proliferation and apoptosis and has been found in a broad variety of cancer cells.|
|Preservative & Stabilisers||0.09% Sodium Azide; 0.01% Bovine Serum Albumin; 50% Glycerol|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-5-Lipoxygenase (pSer523) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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