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>   home   >   Products   >   Primary Antibodies   >   Anti-Rat PAK 1/2/3 (pThr402) Antibody    

Anti-Rat PAK 1/2/3 (pThr402) Antibody

Rabbit Anti-Rat Polyclonal Antibody

  • WB - Anti-Rat PAK 1/2/3 (pThr402) Antibody  ABD11071
    Western blot of rat hippocampal lysate showing phospho-specific immunolabeling of the ~68 to ~70 kDa PAK protein phosphorylated at Thr402
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P35465
Other Accession Q62829, Q64303
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 60578 Da
Additional Information
Purification Antisera to PAK 1/2/3 (pThr402) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns
Immunogen Synthetic phosphopeptide corresponding to amino acid sequence surrounding phosphorylated Thr402 of rat PAK2
Shelf Life 12 months from date of reconstitution.
Gene ID 29431
Other Names Serine/threonine-protein kinase PAK 1,, Alpha-PAK, Protein kinase MUK2, p21-activated kinase 1, PAK-1, p68-PAK, Pak1
Target/Specificity Rabbit anti-Rat PAK 1/2/3 (pThr402) antibody specifically recognizes rat p21-activated kinases (PAK) 1, 2 and 3 when phosphorylated at Thr402. PAKs are a highly conserved family of serine-threonine protein kinases that interact with small GTPases of the RHO family to re-organise the cytoskeleton. Alpha-PAK (PAK1) and beta-PAK (PAK3) are mostly expressed in the brain while gamma-PAK (PAK2) is expressed ubiquitously. In mammals, PAKs regulate the MAPK pathways and cytoskeletal organisation by increasing actin and tubulin polymerisation. PAKs also phosphorylate steroid receptors such as the estrogen and progesterone receptors. In humans the loss of function of PAK-3 is associated with nonsyndromic X-linked mental retardation. PAKs are phosphorylated on multiple residues, Autophosphorylation at Thr402 has been reported to be critical for activation of PAK kinases. It should be noted that Thr402 in PAK2 corresponds to Thr423 in PAK1.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin; 50% Glycerol
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Rat PAK 1/2/3 (pThr402) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD11071
(40 western blots)
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