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Anti-Human Dopamine Transporter (Extracellular Loop 2) Antibody

Rabbit Anti-Human Polyclonal Antibody

  • WB - Anti-Human Dopamine Transporter (Extracellular Loop 2) Antibody  ABD11073
    Wester blot of 10μg of human caudate lysate showing specific immunolablelling of the ~88 kDa DATEL2 protein.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q01959
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 68495 Da
Additional Information
Other Species Pr
Purification Antisera to human dopamine transporter (Extracellular loop 2) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen Keyhole limpet haemocyanin conjugated synthetic peptide from the extracellular loop 2 region of human dopamine transporter.
Shelf Life 18 months from date of despatch.
Gene ID 6531
Other Names Sodium-dependent dopamine transporter, DA transporter, DAT, Solute carrier family 6 member 3, SLC6A3, DAT1
Target/Specificity ABD11073 recognises extracellular loop 2 of the human dopamine transporter (DAT). DAT is a Na+/Cl- dependent transport protein that is responsible for clearing synaptic dopamine by transporting it into neurons. The protein is implicated in a number of dopamine-related disorders such as attention deficit hyperactivity disorder, Parkinson’s disease, depression and drug abuse. DAT consists of 12 transmembrane domains connected by intra- and extracellular loops. The largest, extracellular loop 2, occurs between transmembrane domains 3 and 4 and contains N-linked carbohydrates and a putative disulfide bond. It is the site on the DAT protein that is most sensitive to proteolysis.
Preservative & Stabilisers 0.09% Sodium Azide; 0.01% Bovine Serum Albumin; 50% Glycerol
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Dopamine Transporter (Extracellular Loop 2) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Gaffaney, J. D. and Vaughan, R. A. (2004) Uptake inhibitors but not substrates induce protease resistance in extracellular loop two of the dopamine transporter.
Mol. Pharmacol. 65:692-701.

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Cat# ABD11073
(40 western blots)
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