|Application ||WB, E|
|Calculated MW||70898 Da|
|Purification||Antiserum to human Hsc70 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.|
|Immunogen||Peptide sequence C-EKLQGKINDEDKQK corresponding to the internal region of Hsc70 (NP_006588.1)|
|Shelf Life||18 months from date of despatch.|
|Other Names||Heat shock cognate 71 kDa protein, Heat shock 70 kDa protein 8, Lipopolysaccharide-associated protein 1, LAP-1, LPS-associated protein 1, HSPA8, HSC70, HSP73, HSPA10|
|Target/Specificity||Goat anti-Human Hsc70 antibody recognizes human Heat shock cognate 71 kDa protein, also known as Heat shock 70 kDa protein 8 or Lipopolysaccharide-associated protein 1. Hsc70 is a 646 amino acid ubiquitously expressed cytoplasmic heat-shock cognate protein and member of the heat shock protein 70 family, encoded for by the HSPA8 gene. Goat anti-Human Hsc70 antibody recognizes epitopes in the region between amino acids 555 and 570 of Hsc70 isoform 1, missing in isoform 2.Heat shock proteins are a group of constitutively expressed or heat/stress-inducible proteins which act as molecular chaperones and play an essential role in protein folding/unfolding and transport of proteins across membranes. Hsc70 facilitates the correct folding of newly-synthesized polypeptides (Flynnet al.1989) and also participates in the ATPase disassociation of clathrin-coated vesicles during endocytosis, in conjunction with the co-chaperone auxilin (Jianget al.1997).|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin;|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Hsc70 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
1. Schmid, S.L. (1997) Clathrin-coated vesicle formation and protein sorting: An integrated process. Annu. Rev. Biochem. 66: 511-548.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.