|Application ||WB, E|
|Calculated MW||70898 Da|
|Purification||Antiserum to human Hsc70 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.|
|Immunogen||Peptide sequence C-EKLQGKINDEDKQK corresponding to the internal region of Hsc70 (NP_006588.1)|
|Shelf Life||18 months from date of despatch.|
|Other Names||Heat shock cognate 71 kDa protein, Heat shock 70 kDa protein 8, Lipopolysaccharide-associated protein 1, LAP-1, LPS-associated protein 1, HSPA8, HSC70, HSP73, HSPA10|
|Target/Specificity||Goat anti-Human Hsc70 antibody recognizes human Heat shock cognate 71 kDa protein, also known as Heat shock 70 kDa protein 8 or Lipopolysaccharide-associated protein 1. Hsc70 is a 646 amino acid ubiquitously expressed cytoplasmic heat-shock cognate protein and member of the heat shock protein 70 family, encoded for by the HSPA8 gene. Goat anti-Human Hsc70 antibody recognizes epitopes in the region between amino acids 555 and 570 of Hsc70 isoform 1, missing in isoform 2.Heat shock proteins are a group of constitutively expressed or heat/stress-inducible proteins which act as molecular chaperones and play an essential role in protein folding/unfolding and transport of proteins across membranes. Hsc70 facilitates the correct folding of newly-synthesized polypeptides (Flynnet al.1989) and also participates in the ATPase disassociation of clathrin-coated vesicles during endocytosis, in conjunction with the co-chaperone auxilin (Jianget al.1997).|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin;|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Hsc70 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Schmid, S.L. (1997) Clathrin-coated vesicle formation and protein sorting: An integrated process. Annu. Rev. Biochem. 66: 511-548.
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