|Application ||WB, IF, IP|
|Calculated MW||67919 Da|
|Purification||Antiserum to human TCF4 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.|
|Immunogen||Recombinant human TCF4 expressed in bacteria.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Transcription factor 7-like 2, HMG box transcription factor 4, T-cell-specific transcription factor 4, T-cell factor 4, TCF-4, hTCF-4, TCF7L2, TCF4|
|Target/Specificity||ABD11086 specifically recognises human TCF4 (transcription factor 4), otherwise known as TF7L2, a basic helix-loop-helix (bHLH) domain-containing nuclear protein and member of the Tcf/Lef family, which interacts with beta-catenin to mediate Wnt signalling, acting as a transcriptional activator in cell development processes.ABD11086 is suitable for use in immunofluorescence studies on cells using acetone/methanol fixation.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human TCF4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Valenta, T. et al. (2006) HIC1 attenuates Wnt signaling by recruitment of TCF-4 and Beta-catenin to the nuclear bodies. EMBO J. 25: 2326-2337.
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