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Anti-FLIP Gamma/Delta (C-Terminal) Antibody

Rabbit Anti-Human Polyclonal Antibody

  • ICC - Anti-FLIP Gamma/Delta (C-Terminal) Antibody  ABD11091
    Immunocytochemical staining of HeLa cells with Rabbit anti FLIP gamma/ delta
  • WB - Anti-FLIP Gamma/Delta (C-Terminal) Antibody  ABD11091
    Western blot analysis of total cell lysates from HeLa (H), Jurkat (J), THP-1 (T), A431 (A), K562 and NIH/3T3 cells probed with Rabbit anti FLIP gamma/delta
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O15519
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 55344 Da
Additional Information
Other Species M
Purification Antiserum to human FLIP gamma/delta (CT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen A peptide corresponding to aa191-209 at the C-terminus of human FLIP delta/FLIPs form.
Shelf Life 18 months from date of despatch.
Gene ID 8837
Other Names CASP8 and FADD-like apoptosis regulator, Caspase homolog, CASH, Caspase-eight-related protein, Casper, Caspase-like apoptosis regulatory protein, CLARP, Cellular FLICE-like inhibitory protein, c-FLIP, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, I-FLICE, MACH-related inducer of toxicity, MRIT, Usurpin, CASP8 and FADD-like apoptosis regulator subunit p43, CASP8 and FADD-like apoptosis regulator subunit p12, CFLAR, CASH, CASP8AP1, CLARP, MRIT
Target/Specificity Rabbit anti-FLIP Gamma/Delta antibody recognises the apoptosis related protein designated FLIP (FLICE inhibitory protein). FLIP interacts with the adaptor protein FADD, and with Caspase-8 (FLICE) and Caspase-10 (FLICE2) to inhibit apoptosis induced by death receptors including CD95, DR3, TRAIL-R and TNFR1. FLICE inhibitory proteins were initially identified in virus and human and were accordingly designated as v-FLIPs and c-FLIPs. Four spice variants of c-FLIP have been identified and are classified as FLIP alpha, beta, delta and gamma. Rabbit anti-FLIP Gamma/Delta antibody recognizes human and murine delta and gamma variants but not the alpha or beta, variants. FLIP is widely expressed in human tissues and a diverse range of cell lines.
Preservative & Stabilisers 0.02% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-FLIP Gamma/Delta (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Thome M., et al (1997) Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors.
Nature 386:517-521.2. Irmler M., et al (1997) Inhibition of death receptor signals by cellular FLIP.
Nature 388:190-195.3. Shu H.B., et al (1997) Casper is a FADD- and caspase-related inducer of apoptosis.
Immunity 6:751-763.4. Hu S., et al (1997) I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis.
J. Biol. Chem. 272:17255-17257.

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Cat# ABD11091
(40 western blots)
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