|Application ||WB, IHC-P|
|Calculated MW||53322 Da|
|Purification||Antisera to mouse RIP3 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.|
|Immunogen||Synthetic peptide sequence corresponding to amino acids 473-486 of mouse RIP3.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Receptor-interacting serine/threonine-protein kinase 3, 22.214.171.124, RIP-like protein kinase 3, Receptor-interacting protein 3, RIP-3, mRIP3, Ripk3, Rip3|
|Target/Specificity||Rabbit anti-RIP3 antibody recognizes mouse serine-threonine kinase receptor-interacting protein 3 (RIP3). RIP3 is related to RIP and RIP2 but does not posses a death domain or CARD motif at its C-terminus. RIP3 activates NF-Kappa B and potently induces apoptosis.RIP3 is expressed in a range of tissues including the spleen, liver, testis, heart, brain and lung.|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-RIP3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Günther, C. et al. (2011) Caspase-8 regulates TNF-α-induced epithelial necroptosis and terminal ileitis.Nature. 477: 335-9. 2. Meng L et al. (2015) RIP3-mediated necrotic cell death accelerates systematic inflammation and mortality.Proc Natl Acad Sci U S A. pii: 201514730. 3. Huang, S.C. et al. (2014) Tumor necrosis factor suppresses NR5A2 activity and intestinal glucocorticoid synthesis to sustain chronic colitis.Sci Signal. 7 (314): ra20. 4. Mizumura, K. et al. (2014) Mitophagy-dependent necroptosis contributes to the pathogenesis of COPD.J Clin Invest. 124 (9): 3987-4003. 5. Feng, S. et al. (2007) Cleavage of RIP3 inactivates its caspase-independent apoptosis pathway by removal of kinase domain.Cell Signal. 19 (10): 2056-67.1. Yu, P.W., et al (1999) Identification of RIP3, a RIP-like kinase that activates apoptosis and NF-κB. Curr. Biol. 9:539-422. Sun, X., et al (1999) RIP3, a novel apoptosis-inducing kinase. J Biol Chem. 274:16871-5.3. Pazdernik, N.J., et al (1999) Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-κB. Mol Cell Bio.19:6500-8
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