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Anti-RIP3 Antibody

Rabbit Anti-Mouse Polyclonal Antibody

  • IHC - Anti-RIP3 Antibody  ABD11144
    Immunohistochemical staining of rat kidney with Rabbit anti RIP3
  • WB - Anti-RIP3 Antibody  ABD11144
    Western blot analysis of whole cell lysate from mouse 3T3 cells probed with Rabbit anti RIP3 in the absence (A) or presence (B) of blocking peptide
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9QZL0
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 53322 Da
Additional Information
Other Species Rat
Purification Antisera to mouse RIP3 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Immunogen Synthetic peptide sequence corresponding to amino acids 473-486 of mouse RIP3.
Shelf Life 18 months from date of despatch.
Gene ID 56532
Other Names Receptor-interacting serine/threonine-protein kinase 3,, RIP-like protein kinase 3, Receptor-interacting protein 3, RIP-3, mRIP3, Ripk3, Rip3
Target/Specificity Rabbit anti-RIP3 antibody recognizes mouse serine-threonine kinase receptor-interacting protein 3 (RIP3). RIP3 is related to RIP and RIP2 but does not posses a death domain or CARD motif at its C-terminus. RIP3 activates NF-Kappa B and potently induces apoptosis.RIP3 is expressed in a range of tissues including the spleen, liver, testis, heart, brain and lung.
Preservative & Stabilisers 0.02% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-RIP3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Günther, C. et al. (2011) Caspase-8 regulates TNF-α-induced epithelial necroptosis and terminal ileitis.
Nature. 477: 335-9. 2. Meng L et al. (2015) RIP3-mediated necrotic cell death accelerates systematic inflammation and mortality.
Proc Natl Acad Sci U S A. pii: 201514730. 3. Huang, S.C. et al. (2014) Tumor necrosis factor suppresses NR5A2 activity and intestinal glucocorticoid synthesis to sustain chronic colitis.
Sci Signal. 7 (314): ra20. 4. Mizumura, K. et al. (2014) Mitophagy-dependent necroptosis contributes to the pathogenesis of COPD.
J Clin Invest. 124 (9): 3987-4003. 5. Feng, S. et al. (2007) Cleavage of RIP3 inactivates its caspase-independent apoptosis pathway by removal of kinase domain.
Cell Signal. 19 (10): 2056-67.1. Yu, P.W., et al (1999) Identification of RIP3, a RIP-like kinase that activates apoptosis and NF-κB.
Curr. Biol. 9:539-422. Sun, X., et al (1999) RIP3, a novel apoptosis-inducing kinase.
J Biol Chem. 274:16871-5.3. Pazdernik, N.J., et al (1999) Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-κB.
Mol Cell Bio.19:6500-8

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Cat# ABD11144
(40 western blots)
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