|Application ||WB, IHC-P|
|Calculated MW||129931 Da|
|Purification||Antisera to human NogoA were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.|
|Immunogen||Synthetic peptide sequence corresponding to a 23 amino acid sequence from near the carboxy terminus of Human NogoA|
|Shelf Life||18 months from date of despatch.|
|Other Names||Reticulon-4, Foocen, Neurite outgrowth inhibitor, Nogo protein, Neuroendocrine-specific protein, NSP, Neuroendocrine-specific protein C homolog, RTN-x, Reticulon-5, RTN4, KIAA0886, NOGO|
|Target/Specificity||Rabbit anti-Human NOGOA antibody recognizes human Reticulon-4 (RTN4), also known as NogoA, neurite outgrowth inhibitor, Foocen, Neuroendocrine-specific protein, Neuroendocrine-specific protein C homolog, Reticulon-5 or RTN-x. NogoA is a 1192 amino acid multi pass transmembrane protein associated with the endoplasmic reticulum, a member of a family of integral membrane proteins termed reticulons. Six isoforms of NogoA can be generated by alternative splicing, the canonical isoform 1 is predominantly expressed in the brain and testis with weaker expression in the heart and skeletal muscle.Reticulons are involved in various neurodegenerative diseases such as Amyotrophic lateral sclerosis,and multiple sclerosis (Chiurchiùet al.2014). Reticulon proteins have been demonstrated to regulate many cellular processes and interact with multiple proteins and receptors such as BACE. NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor (Niederöstet al.2002). NogoA is highly expressed in oligodendrocytes in the white matter of the CNS (Kuhlmannet al.2008). Blocking NogoA activity with antibodies or other factors results in improved long distance axonal regeneration and functional recovery in experimental CNS lesion models (Schwab 2004).NOGOA has a predicted molecular weight of 130kDa however, despite its predicted molecular weight, NogoA typically migrates at ~180kDa in an SDS-PAGE. Rabbit anti-human NOGOA antibody is expected to recognize all isoforms of NogoA.|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human NogoA (N-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Dann, A. et al. (2011) Cytosolic RIG-I-like helicases act as negative regulators of sterile inflammation in the CNS.Nat Neurosci. 15: 98-106. 2. Gerondopoulos, A. et al. (2014) Rab18 and a Rab18 GEF complex are required fornormal ER structureJ Cell Biol. pii: jcb.201403026. 1. Yan, R. et al. (2006) Reticulon proteins: emerging players in neurodegenerative diseases.Cell Mol Life Sci. 63 (7-8): 877-89.2. Schweigreiter, R. & Bandtlow, C.E. (2006) Nogo in the injured spinal cord.J Neurotrauma. 23 (3-4): 384-96.3. Dupuis, L. et al. (2002) Nogo provides a molecular marker for diagnosis of amyotrophic lateral sclerosis.Neurobiol Dis. 10 (3): 358-65.4. Chen, M.S. et al. (2000) Nogo-A is a myelin-associated neurite outgrowth inhibitor and an antigen for monoclonal antibody IN-1.Nature. 403 (6768): 434-9.
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