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Anti-AIF1 (C-Terminal) Antibody

Goat Anti-Human Polyclonal Antibody

  • WB - Anti-AIF1 (C-Terminal) Antibody  ABD11200
    Western blot analysis of rat brain lysate probed with Goat anti Human AIF1 followed by HRP conjugated anti Goat antibody and detection by chemiluminescence
  • IHC-P - Anti-AIF1 (C-Terminal) Antibody  ABD11200
    Immunohistochemical staining of formalin fixed, paraffin embedded mouse brain with Goat anti Human AIF1 antibody following heat mediated antigen retrieval in Tris/EDTA buffer (pH9.0)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P55009
Other Accession P55008
Reactivity Human
Host Goat
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 16827 Da
Additional Information
Other Species M,Pig,Rat
Purification Antiserum to human AIF1 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Immunogen Synthetic peptide sequence C-TGPPAKKAISELP from the C-terminal region of AIF1 (NP_116573.1; NP_001614.3).
Shelf Life 18 months from date of despatch.
Gene ID 29427
Other Names Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, MRF-1, Microglia response factor, Aif1, Iba1, Mrf1
Target/Specificity Goat anti-AIF1 antibody recognizes human allograft inflammatory factor 1 (AIF1), otherwise known as Ionized calcium-binding adapter molecule 1 (IBA1). AIF1 is a 147 amino acid ~17kDa actin-binding protein involved in macrophage activation, vascular smooth muscle cell proliferation and activation and T-lymphocyte proliferation (UniProt P55008). Goat anti-AIF1 antibody recognizes epitopes at the C-terminal region of AIF1 and is expected to recognize AIF1 isoform 1 and isoform 3 formed by alternative splicing.AIF1 was first identified in rat cardiac allografts with chronic rejection (Utanset al.1995), is expressed by activated monocytes and macrophages (Orsmarket al.2007) and plays a significant role in vascular inflammation (Chenet al.2004)Goat anti-AIF1 has been used successfully for the identification of AIF1 expressing cells in an inflammatory model in formalin fixed mouse brain following heat mediated antigen retrieval with sodium citrate buffer by immunohistochemistry (Wanget al.2014).
Preservative & Stabilisers 0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin;
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-AIF1 (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Wang, X. et al. (2014) Pseudoginsenoside-F11 (PF11) exerts anti-neuroinflammatory effects on LPS-activated microglial cells by inhibiting TLR4-mediated TAK1/IKK/NF-κB, MAPKs and Akt signaling pathways.
Neuropharmacology. 79: 642-56.

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Cat# ABD11200
(40 western blots)
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