|Application ||WB, IHC-P, E|
|Calculated MW||16827 Da|
|Purification||Antiserum to human AIF1 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.|
|Immunogen||Synthetic peptide sequence C-TGPPAKKAISELP from the C-terminal region of AIF1 (NP_116573.1; NP_001614.3).|
|Shelf Life||18 months from date of despatch.|
|Other Names||Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, MRF-1, Microglia response factor, Aif1, Iba1, Mrf1|
|Target/Specificity||Goat anti-AIF1 antibody recognizes human allograft inflammatory factor 1 (AIF1), otherwise known as Ionized calcium-binding adapter molecule 1 (IBA1). AIF1 is a 147 amino acid ~17kDa actin-binding protein involved in macrophage activation, vascular smooth muscle cell proliferation and activation and T-lymphocyte proliferation (UniProt P55008). Goat anti-AIF1 antibody recognizes epitopes at the C-terminal region of AIF1 and is expected to recognize AIF1 isoform 1 and isoform 3 formed by alternative splicing.AIF1 was first identified in rat cardiac allografts with chronic rejection (Utanset al.1995), is expressed by activated monocytes and macrophages (Orsmarket al.2007) and plays a significant role in vascular inflammation (Chenet al.2004)Goat anti-AIF1 has been used successfully for the identification of AIF1 expressing cells in an inflammatory model in formalin fixed mouse brain following heat mediated antigen retrieval with sodium citrate buffer by immunohistochemistry (Wanget al.2014).|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin;|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-AIF1 (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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1. Wang, X. et al. (2014) Pseudoginsenoside-F11 (PF11) exerts anti-neuroinflammatory effects on LPS-activated microglial cells by inhibiting TLR4-mediated TAK1/IKK/NF-κB, MAPKs and Akt signaling pathways.Neuropharmacology. 79: 642-56.
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