|Application ||WB, IF, ID|
|Calculated MW||29246 Da|
|Purification||Antisera to human carbonic anhydrase II were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.|
|Immunogen||Purified human carbonic anhydrase II (CAII) prepared from erythrocytes.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Carbonic anhydrase 2, 220.127.116.11, Carbonate dehydratase II, Carbonic anhydrase C, CAC, Carbonic anhydrase II, CA-II, CA2|
|Target/Specificity||Sheep anti-Human carbonic anhydrase II antibody recognizes human carbonic anhydrase II, also known as Carbonate dehydratase II orCarbonic anhydrase C. Carbonic anhydrase II is a 259 amino acid ~30 kDa enzyme essential for bone resorption and osteoclast differrentiation.Mutations in the CA2 gene has been identified as the cause of Osteopetrosis, autosomal recessive 3 (OPTB3), a rare disease characterized by particularly dense bone, cerebral calcification and renal tubular acidisis (Shahet al2004).Product identity is confirmed by double diffusion vs human CAII. No reactivity is seen in immunodiffusion against CAI.|
|Preservative & Stabilisers||0.09% Sodium Azide; 0.1% EACA; 1mM EDTA; 0.01% Benzamidine|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Carbonic Anhydrase II Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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1. Toye, A.M. et al. (2002) Band 3 Walton, a C-terminal deletion associated with distal renal tubular acidosis, is expressed in the red cell membrane but retained internally in kidney cells.Blood. 99 (1): 342-7. 2. Garcia, A.D. et al. (2010) Sonic hedgehog regulates discrete populations of astrocytes in the adult mouse forebrain.J Neurosci. 30: 13597-608. 3. Doyle, K.P. et al.. (2010) TGFβ signaling in the brain increases with aging and signals to astrocytes and innate immune cells in the weeks after stroke.J Neuroinflammation. 7: 62. 4. Alvarez, B.V. et al. (2005) Metabolon disruption: a mechanism that regulates bicarbonate transport.EMBO J. 24: 2499-511. 5. Tzartos, J.S. et al. (2008) Interleukin-17 production in central nervous system-infiltrating T cells and glial cells is associated with active disease in multiple sclerosis.Am J Pathol. 172: 146-55. 6. Sudarov, A. et al. (2011) Ascl1 genetics reveals insights into cerebellum local circuit assembly.J Neurosci. 31: 11055-69. 7. Salman, S. et al. (2013) Chronic exposure of neonatal rat adrenomedullary chromaffin cells to opioids in vitro blunts both hypoxia and hypercapnia chemosensitivity.J Physiol. 591 (Pt 2): 515-29. 8. Salman, S. et al. (2014) Chronic opioids regulate KATP channel subunit Kir6.2 and carbonic anhydrase I and II expression in rat adrenal chromaffin cells via HIF-2a and protein kinase A.Am J Physiol Cell Physiol. 307: C266-77.
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