|Application ||WB, E|
|Other Accession||Q61483, P97677|
|Calculated MW||78056 Da|
|Purification||Antiserum to human DLL1 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.|
|Immunogen||Synthetic peptide sequence C-ATQRHLTVGEEWSQD from the internal region of DLL1 (NP_005609.3).|
|Shelf Life||18 months from date of despatch.|
|Other Names||Delta-like protein 1, Drosophila Delta homolog 1, Delta1, H-Delta-1, DLL1|
|Target/Specificity||Goat anti-Human Delta-like protein 1 antibody specifically recognises Delta-like protein 1 (DLL1), one of the five major ligands of the Notch signalling pathway, which is activated through the binding of specific ligands to the Notch receptors Notch 1-4.The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumour progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation.DLL1 is widely expressed, and acts as a mediator of cell fate decisions during haematopoiesis, and may play a role in cell-to-cell communication in mammalian embryos. DLL1 plays an important role in B and T cell differentiation, in embryonic somite formation and patterning, and associates with the scaffolding protein MAGI1 at adherens junctions on neuronal processes. Signalling through DLL1 and Notch 2 has been implicated in the development of marginal zone B cells (MZB).|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin;|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Delta-Like Protein 1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.
Request Quote Here