|Application ||WB, E|
|Calculated MW||108054 Da|
|Purification||Antiserum to human MAML1 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.|
|Immunogen||Synthetic peptide sequence VLPTCPMAEFALPR from the N-Terminal region of MAML1 (NP_055572.1).|
|Shelf Life||18 months from date of despatch.|
|Other Names||Mastermind-like protein 1, Mam-1, MAML1 (HGNC:13632)|
|Target/Specificity||Rabbit anti-Human MAML1 antibody recognises an epitope within the N-Terminal (NT) region of human MAML1 (Mastermind-like Protein 1), a widely expressed nuclear protein and member of the mastermind family, which acts as a crucial coactivator of Notch-mediated transcriptional activation, and plays a role in hematopoietic development, by acting as a regulator of Notch-mediated lymphoid cell fate decisions. MAML1 interacts with the Mediator complex component CDK8 (Cyclin-dependent kinase 8), enhancing the phosphorylation and proteolytic turnover of the Notch intracellular domain (NICD) within the nucleus, and forms a DNA-binding complex with RBPSUH/RBP-J kappa/CBF1, playing a vital role in the transcriptional activation of Notch targets. Studies have shown that the NT region of MAML1 interacts with the DNA-binding domain of the tumor suppressor protein p53, and that over-expression of MAML1, greatly increases p53-mediated gene transcription and biological function.|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin;|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human MAML1 (N-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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