|Application ||WB, E|
|Calculated MW||11367 Da|
|Purification||Antisera to acetylated histone H4 were raised by repeated immunisation of rabbits with highly purified antigen.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Histone H4, HIST1H4A, H4/A, H4FA|
|Target/Specificity||Histone H4 acetylated at lysine 12|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Histone H4 (Ac12) Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Turner, B.M. et al. (1989) Histone H4 acetylation in human cells. Frequency of acetylation at different sites defined by immunolabelling with site-specific antibodies.FEBS Letters 253: 141-145. 2. Turner, B.M. et al. (1992) Histone H4 isoforms acetylated at specific lysine residues define individual chromosomes and chromatin domains in Drosophila polytene nuclei.Cell 69: 375-384. 3. Belyaev, N.D. et al. (1996) Differential underacetylation of histones H2A, H3 and H4 on the inactive X chromosome in human female cells.Hum. Genet. 97: 573-578. 4. Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.J Inflamm (Lond). 8: 1. 5. Chen, W.Y. et al. (2000) Molecular mechanism for silencing virally transduced genes involves histone deacetylation and chromatin condensation.Proc Natl Acad Sci U SA. 97: 377-82. 6. Friis, R.M. et al. (2009) A glycolytic burst drives glucose induction of global histone acetylation by picNuA4 and SAGA.Nucleic Acids Res. 37: 3969-80. 7. Ito, K. et al. (2000) Glucocorticoid receptor recruitment of histone deacetylase 2 inhibits interleukin-1beta-induced histone H4 acetylation on lysines 8 and 12.Mol Cell Biol. 20: 6891-903. 8. Kobor, M.S. et al (2004) A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin.PLoS Biol. 2: E131. 9. Lin, X. et al. (2001) Reversal of GSTP1 CpG island hypermethylation and reactivation of pi-class glutathione S-transferase (GSTP1) expression in human prostate cancer cells by treatment with procainamide.Cancer Res. 61: 8611-6. 10. Nie, M. et al. (2003) Transcriptional regulation of cyclooxygenase 2 by bradykinin and interleukin-1beta in human airway smooth muscle cells: involvement of different promoter elements, transcription factors, and histone h4 acetylation.Mol Cell Biol. 23: 9233-44. 11. Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.J Inflamm (Lond). 8: 1.
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