|Application ||WB, IHC-P|
|Other Accession||P12036, P16884|
|Calculated MW||116994 Da|
|Purification||Antiserum to bovine NFH was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.|
|Immunogen||GST-tagged bovine NFH recombinant protein.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein, Nefh, Kiaa0845, Nfh|
|Target/Specificity||Rabbit anti-Bovine Neurofilament H 200kDa antibody recognizes bovine intermediate 200kDa Neurofilament H (NFH), a 1026 amino acid ~200kDa protein involved in the maintenence of neuronal integrity.Neurofilaments are a major component of the cellular cytoskeleton, acting as the most abundant support for the axon cytoplasm. They exist as three neurofilament proteins: 68/70kDa (NFL) 160kDa (NFM) and 200kDa (NFH), which are expressed in both the central and peripheral nervous systems. Although typically expressed by neurons, neurofilaments are also expressed by neuroblastomas, neuromas, gangliogliomas, ganglioneuromas, and ganglioneuroblastomas, as well as paragangliomas, carcinoids, skin neuroendocrine carcinomas, and oat cell carcinomas of the lung (Lehtoet al.1983).Defects in NFH are responsible for susceptibility to the neurodegenerative disorder amyotrophic lateral sclerosis (ALS) which affects upper and lower motor neurons, and results in fatal paralysis (Mendonçaet al.2005).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Bovine Neurofilament H 200kDa Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Kaneko, S. et al. (2006) Protecting axonal degeneration by increasing nicotinamide adenine dinucleotide levels in experimental autoimmune encephalomyelitis models.J Neurosci. 26: 9794-804. 2. Li, Y. et al. (2008) Transplanted olfactory ensheathing cells incorporated into the optic nerve head ensheathe retinal ganglion cell axons: possible relevance to glaucoma.Neurosci Lett. 440: 251-4. 3. Ignatov, A. et al. (2003) Role of the G-protein-coupled receptor GPR12 as high-affinity receptor for sphingosylphosphorylcholine and its expression and function in brain development.J Neurosci. 23: 907-14. 4. Leypoldt, F. et al. (2002) Neuronal differentiation of cultured human NTERA-2cl.D1 cells leads to increased expression of synapsins.Neurosci Lett. 2002 May 10;324(1):37-40. 5. Savolainen, H. et al. (2009) Cortical demyelination in PML and MS: similarities and differences.Neurology. 72: 861. 6. Zhang, Z. et al. (2011) The role of single cell derived vascular resident endothelial progenitor cells in the enhancement of vascularization in scaffold-based skin regeneration.Biomaterials. 32: 4109-17. 1. Liu, Q. et al. (2004) Neurofilament proteins in neurodegenerative diseases.Cell. Mol. Life Sci.61: 3057-3075.
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