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Anti-Human Mammaglobin A Antibody

Goat Anti-Human Polyclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13296
Reactivity Human
Host Goat
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 10499 Da
Additional Information
Purification Antiserum to Human mammaglobin A was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography using the immunizing peptide
Immunogen Synthetic peptide sequence C-NPQVSKTEYKEL from the internal region of Human mammaglobin A (NP_002402.1)
Shelf Life 18 months from date of despatch.
Gene ID 4250
Other Names Mammaglobin-A, Mammaglobin-1, Secretoglobin family 2A member 2, SCGB2A2, MGB1, UGB2
Target/Specificity Goat anti-Human mammoglobin A antibody recognizes human mammaglobin A, also known as Secretoglobin family 2A member 2, the product of the SCGB2A2 gene. Mammaglobin A is a ~10.5kDa protein expressed in mammary tissue (Watsonet al.2001).Immunohistochemical studies have shown an increase in the sensitivity of breast cancer detection using mammaglobin antibodies, when compared to BRST-1 and GCDFP-15, and that mammaglobin can be utilised to confirm breast origin of metastic tumors in axillary lymph nodes (ALNs), which are used as an important prognostic factor for staging breast cancer, and subsequent treatment (Layqueet al.1999).The detection of circulating tumor cells is a useful monitoring tool for the progression of micro-metastases in breast cancer patients with assays based primarily on reverse-transcriptase PCR having been developed (Zachet al.1999,Ookaet al.2001).The mRNA of human mammaglobin has been found to be the most specific marker for the hematogenous spread of breast cancer cells. Since it is cells from primary breast tumors which are normally present in ALNs, distinguishing between breast epithelial cells and lymphoid cells is beneficial and studies using gene expression analysis have determined that mRNA encoding mammaglobin A protein can distinguish between these cells types.Mammaglobin A, a highly specific biomarker for breast tissue, was originally thought to be absent from tumors of non-breast origin. More recent evidence using immunohistochemical studies suggests that the co-expression of mammaglobin A with lipophilin B (SCGB1D2), can be detected in both normal and malignant tissues of the ovary, cervix and uterus (Bignottiet al.2013).Goat anti-human mammoglobin A exhibits staining of cytoplasm in some acinar cells of human breast tissue in paraffin sections. This polyclonal antibody is expected to recognize both mammaglobin isoforms listed in UniProt entry Q13296
Preservative & Stabilisers 0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin;
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Mammaglobin A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD11269
(40 western blots)
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