|Application ||WB, IHC-P, IF, E|
|Calculated MW||39645 Da|
|Purification||Antiserum to Wnt-3 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography using the immunizing peptide|
|Immunogen||Synthetic peptide sequence C-GRGHNTRTEKRKEK from the internal region of Wnt-3 (NP_110380.1).|
|Shelf Life||18 months from date of despatch.|
|Other Names||Proto-oncogene Wnt-3, Proto-oncogene Int-4 homolog, WNT3, INT4|
|Target/Specificity||Goat anti-Wnt-3 antibody recognises both human and mouse proto-oncogene Wnt-3, a member of the Wnt family, and ligand of the frizzled family of seven transmembrane receptors.Wnt-3, otherwise known as Int-4, is involved in cell fate decisions and patterning during embryogenesis, cell signalling during neural tube development, and implicated in the tumorogenesis of several cancers including breast, lung, hepatocellular(HCC), and gastric carcinoma.Over-expression of Wnt-3 playes a pivotal role in the resistance of HER2-overexpressing breast tumors to the breast cancer treatment drug Trastuzumab, also known as Herceptin (Wuet al.2012). The effects of Wnt-3 over-expression included the transactivated expression of EGFR (epidermal growth factor receptor) an increased expression of B-catenin within the nucleus, an increase in N-cadherin and the neural crest transcription factor SLUG, as well as promoting an EMT (epithelial to mesenchymal transition) response. Goat anti-Wnt-3 polyclonal antibody exhibits strong fibrous staining in myoepithelial cells of human breast tissue in paraffin sections. Goat anti-Wnt-3 polyclonal antibody has been shown to stain mouse hippocampal primary astrocytes in ICC/IF studies, which investigated the role of Wnt-3 levels and Wnt-3 expressing astrocytes, in the regulation of age-associated decline in adult neurogenesis (Okamotoet al.2011).|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin;|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-WNT-3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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