|Application ||WB, IF, E, IP, GS|
|Calculated MW||60219 Da|
|Purification||Antisera to NFkB p65 were raised by repeated immunisation of rabbits with highly purified antigen.|
|Immunogen||NFkB p65 peptide corresponding to the C-terminus region of the human protein conjugated to KLH.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, NFKB3|
|Target/Specificity||Rabbit anti-Human NFkB p65 antibody recognizes the human Transcription factor p65, also known as Nuclear factor NF-kappa-B p65 subunit, NFκB p65, RelA or Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3. NFκB p65 is a 551 amino acid ~65 kDa subunit of the NFκB transcription factor present in most cell types and is involved as the endpoint in an array of signal transduction events related to many processes including apotosis, immunity, inflammation and tumorigenesis. The most abundant form of NFκB appears to be the p65-p50 heterodimer. In the active form NFκB p65 is found in the nucleus co-localizing with the ATP-dependent RNA helicase DDX1. NFκB p65 is also found in an inactive form in the cytoplasm associated with the inhibitor IκB (UniProt: Q04206)In a gel supershift assay Rabbit anti-Human NFκB p65 antibody was found to be active against all p65 containing human, mouse or rat NFκB complexes using 0.5ul to 1.0ul per assay. The binding of Rabbit anti-Human NFκB p65 antibody to the NFκB p65 subunit can be blocked using the NFκB p65 control peptide (PHP073).|
|Preservative & Stabilisers||0.01% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-NFkB p65 Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Cruz, M.T. et al. (1999) Involvement of JAK2 and MAPK on type II nitric oxide synthase expression in skin-derived dendritic cells.Am J Physiol. 277: C1050-7. 2. Parmentier. M, et al. (2000) Regulation of lipopolysaccharide-mediated interleukin-1beta release by N-acetylcysteine in THP-1 cells.Eur. Respir. J. 16: 933-9. 3. Gründker, C. et al. (2000) Luteinizing hormone-releasing hormone induces nuclear factor kappaB-activation and inhibits apoptosis in ovarian cancer cells.J Clin Endocrinol Metab. 85: 3815-20. 4. Sun, J. et al. (2007) Neuropeptide substance P upregulates chemokine and chemokine receptor expression in primary mouse neutrophils.Am J Physiol Cell Physiol. 293: C696-704. 5. Wang, J. et al. (2014) TL1-A can engage death receptor-3 and activate NF-kappa B in endothelial cells.BMC Nephrol. 15: 178. 6. Abdel-Latif, M. (2015) Diethylcarbamazine citrate ameliorates insulin resistance in high-fat diet-induced obese mice via modulation of adipose tissue inflammation.Int Immunopharmacol. : . 7. Takada Y et al. (2009) Odoroside A and ouabain inhibit Na+/K+-ATPase and prevent NF-kappaB-inducible protein expression by blocking Na+-dependent amino acid transport.Biochem Pharmacol. 78 (9): 1157-66. 8. Zhang H et al. (2007) Hydrogen sulfide acts as an inflammatory mediator in cecal ligation and puncture-induced sepsis in mice by upregulating the production of cytokines and chemokines via NF-kappaB.Am J Physiol Lung Cell Mol Physiol. 292 (4): L960-71.
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