|Application ||WB, E, IP, GS|
|Calculated MW||36877 Da|
|Purification||Antisera to NF-Y were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.|
|Immunogen||NF-Y (A subunit) peptide corresponding to a region near the N-terminus conjugated to Keyhole Limpet Hemocyanin.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Nuclear transcription factor Y subunit alpha, CAAT box DNA-binding protein subunit A, Nuclear transcription factor Y subunit A, NF-YA, NFYA|
|Target/Specificity||Rabbit anti-NF-Y antibody recognizes human Nuclear transcription factor Y subunit alpha, also known as NF-Y subunit a, NF-YA or CAAT box DNA-binding protein subunit A. Rabbit anti-NF-Y antibody recognizes both differentially spliced 35 kDa and 42 kDa forms of the A subunit of NF-Y but demonstrates no reactivity against the 25 kDa B subunit.|
|Preservative & Stabilisers||0.01% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-NF-Y Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Nenoi, M. et al. (2001) Regulation of the catalase gene promoter by Sp1, CCAAT-recognizing factors, and a WT1/Egr-related factor in hydrogen peroxide-resistant HP100 cells.Cancer. Res. 61: 5885-94. 2. Paradkar, P.N. and Roth, J.A. (2007) Expression of the 1B isoforms of divalent metal transporter (DMT1) is regulated by interaction of NF-Y with a CCAAT-box element near the transcription start site.J Cell Physiol. 211: 183-8. 3. Petrie, J.L. et al. (2012) Glucose Induces Protein-Targeting-to-Glycogen in Hepatocytes by Fructose 2,6-bisphosphate mediated recruitment of MondoA to the Promoter.Mol Cell Biol. 33: 725-. 4. Gromnicova, R. et al. (2012) Transcriptional control of the multi-drug transporter ABCB1 by transcription factor Sp3 in different human tissues.PLoS One. 7 (10): e48189.
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