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Anti-Inducible Nitric Oxide Synthase Antibody (Serum)

Rabbit Anti-Mouse Polyclonal Antibody

  • WB - Anti-Inducible Nitric Oxide Synthase Antibody (Serum) ABD11276
    Published customer image:Rabbit anti Human nitric oxide synthase antibody used for the detection of iNOS in stimulated microglial lysates by western blotting.Image caption:Inhibition of iNOS and COX-2 expression in LPS-stimulated microglial cells by Af extract. Microglial cells were treated with 100 µg/mL of Af extract, followed by stimulation with LPS for 20 h. Equal amounts of cell lysates were separated by SDS-PAGE and immunoblotted with antibodies to iNOS, COX-2, and β-actin. A. The immunoblot represents one of three different experiments with similar results. B. Data represent the means ± SEM of three independent experiments (n = 3). The levels of each protein were normalized to the levels of β-actin protein.From: Elmann A, Mordechay S, Erlank H, Telerman A, Rindner M, Ofir R. Anti-neuroinflammatory effects of the extract of Achillea fragrantissima.BMC Complement Altern Med. 2011 Oct 21;11:98.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P29477
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 130575 Da
Additional Information
Other Species Rat,H
Purification Antisera to inducible Nitric Oxide Synthase were raised by repeated immunisation of rabbits with highly purified antigen. Whole serum is supplied.
Immunogen Mouse macrophage NOS C-terminal peptide (1131-1144) + additional N-terminal Cys conjugated to KLH (CKKGSALEEPKATRL).
Shelf Life 18 months from date of despatch.
Gene ID 18126
Other Names Nitric oxide synthase, inducible,, Inducible NO synthase, Inducible NOS, iNOS, Macrophage NOS, MAC-NOS, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, Nos2, Inosl
Target/Specificity Rabbit anti-inducible nitric oxide synthase antibody recognizes inducible nitric oxide sythase (iNOS), also known as Macrophage NOS, NOS type II or Peptidyl-cysteine S-nitrosylase NOS2. Rabbit anti-inducible nitric oxide synthase antibody does not cross react with endothelial nitric oxide synthase (eNOS) or brain nitric oxide synthase (bNOS).
Preservative & Stabilisers 0.05% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Inducible Nitric Oxide Synthase Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Lyons, C. et al. (1992) Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line.
J. Biol. Chem. 267: 6370 - 6374. 2. Lowenstein, C. et al. (1992) Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme.
Proc. Natl. Acad. Sci. USA. 89: 6711. 3. Bredt, D. et al. (1991) Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase.
Nature. 351:714-718 4. Hohenstein, B. et al. (2007) Analysis of NO-synthase expression and clinical risk factors in human diabeticnephropathy.
Nephrol Dial Transplant. 23: 1346-54. 5. Fenoglio, C. et al. (2004) Expression of natriuretic peptides, nitric oxide synthase, and guanylate cyclase activity in frog mesonephros during the annual cycle.
Gen Comp Endocrinol. 137: 166-76. 6. Elmann, A. et al. (2011) Anti-neuroinflammatory effects of the extract of Achillea fragrantissima.
BMC Complement Altern Med. 11: 98.

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Cat# ABD11276
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