|Application ||WB, IHC-F|
|Calculated MW||130575 Da|
|Purification||Antisera to inducible Nitric Oxide Synthase were raised by repeated immunisation of rabbits with highly purified antigen. Whole serum is supplied.|
|Immunogen||Mouse macrophage NOS C-terminal peptide (1131-1144) + additional N-terminal Cys conjugated to KLH (CKKGSALEEPKATRL).|
|Shelf Life||18 months from date of despatch.|
|Other Names||Nitric oxide synthase, inducible, 188.8.131.52, Inducible NO synthase, Inducible NOS, iNOS, Macrophage NOS, MAC-NOS, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, Nos2, Inosl|
|Target/Specificity||Rabbit anti-inducible nitric oxide synthase antibody recognizes inducible nitric oxide sythase (iNOS), also known as Macrophage NOS, NOS type II or Peptidyl-cysteine S-nitrosylase NOS2. Rabbit anti-inducible nitric oxide synthase antibody does not cross react with endothelial nitric oxide synthase (eNOS) or brain nitric oxide synthase (bNOS).|
|Preservative & Stabilisers||0.05% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Inducible Nitric Oxide Synthase Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Lyons, C. et al. (1992) Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line.J. Biol. Chem. 267: 6370 - 6374. 2. Lowenstein, C. et al. (1992) Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme.Proc. Natl. Acad. Sci. USA. 89: 6711. 3. Bredt, D. et al. (1991) Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase.Nature. 351:714-718 4. Hohenstein, B. et al. (2007) Analysis of NO-synthase expression and clinical risk factors in human diabeticnephropathy. Nephrol Dial Transplant. 23: 1346-54. 5. Fenoglio, C. et al. (2004) Expression of natriuretic peptides, nitric oxide synthase, and guanylate cyclase activity in frog mesonephros during the annual cycle.Gen Comp Endocrinol. 137: 166-76. 6. Elmann, A. et al. (2011) Anti-neuroinflammatory effects of the extract of Achillea fragrantissima.BMC Complement Altern Med. 11: 98.
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