|Application ||WB, IHC-P, IP, GS|
|Calculated MW||469089 Da|
|Purification||Antisera to DNA-PKcs were raised by repeated immunisation of rabbit with highly purified antigen. Whole serum is supplied.|
|Immunogen||Human DNA-PKcs fragment.|
|Shelf Life||18 months from date of despatch.|
|Other Names||DNA-dependent protein kinase catalytic subunit, DNA-PK catalytic subunit, DNA-PKcs, 18.104.22.168, DNPK1, p460, PRKDC, HYRC, HYRC1|
|Target/Specificity||Rabbit anti-DNA PKcs antibody recognizes the human DNA-dependent protein kinase catalytic subunit, also known as DNA-PKcs, DNPK1 or p460. DNA PKcs is a 4128 amino acid ~460 kDa nuclear protein encoded by the PRKDC gene, acting as a molecular sensor for DNA damage. Mutation of the PRKDC gene can result in Immunodeficiency 26 with or without neurologic abnormalities (IMD26) (van der Burg et al.2009).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-DNA PKcs Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Finnie, N.J. et al. (1995) DNA-dependent protein kinase activity is absent in xrs-6 cells: implications for site-specific recombination and DNA double-strand break repair.Proc Natl Acad Sci U S A. 92 (1): 320-4. 2. Jayaram, S. et al. (2008) E1B 55k-independent dissociation of the DNA ligase IV/XRCC4 complex by E4 34k during adenovirus infection. Virology.382: 163-70. 3. Hoek, M. et al. (2011) An analysis of CAF-1-interacting proteins reveals dynamic and direct interactions with the KU complex and 14-3-3 proteins.J Biol Chem. 286 (12): 10876-87. 4. Frasca, D. et al. (2001) Role of DNA-dependent protein kinase in recognition of radiation-induced DNA damage in human peripheral blood mononuclear cells.Int Immunol. 13: 791-7. 5. Jayaram, S. et al. (2008) Loss of DNA ligase IV prevents recognition of DNA by double-strand break repair proteins XRCC4 and XLF.Nucleic Acids Res. 36: 5773-86. 6. Koike, M. et al. (1999) Differential subcellular localization of DNA-dependent protein kinase components Ku and DNA-PKcs during mitosis.J Cell Sci. 112: 4031-9. 7. Yee, M.C. et al. (2005) A cell-permeable, activity-based probe for protein and lipid kinases.J Biol Chem. 280: 29053-9. 8. Zhao, H.J. et al. (2000) DNA-dependent protein kinase activity correlates with Ku70 expression and radiation sensitivity in esophageal cancer cell lines.Clin Cancer Res. 6: 1073-8. 9. Sirzen, F. et al. (1999) DNA-dependent protein kinase content and activity in lung carcinoma cell lines: correlation with intrinsic radiosensitivity.Eur J Cancer. 35: 111-6. 10. Frasca, D. et al. (2002) The DNA repair protein ku is involved in gp130-mediated signal transduction events in PBMC from young but not from elderly subjects.Exp Gerontol. 37: 321-8. 11. Scarpaci, S. et al. (2003) DNA damage recognition and repair capacities in human naïve and memory T cells from peripheral blood of young and elderly subjects.Mech Ageing Dev. 124: 517-24. 12. Kamachi, M. et al. (2002) Human autoimmune sera as molecular probes for the identification of an autoantigen kinase signaling pathway.J Exp Med. 196: 1213-25. 13. Gaymes, T.J. et al. (2006) Histone deacetylase inhibitors (HDI) cause DNA damage in leukemia cells: a mechanism for leukemia-specific HDI-dependent apoptosis?Mol Cancer Res. 4 (8): 563-73. 14. Koike, M. et al. (1999) Differential subcellular localization of DNA-dependent protein kinase components Ku and DNA-PKcs during mitosis.J Cell Sci. 112 ( Pt 22): 4031-9. 15. Nilsson, A. et al. (1999) Cell cycle-dependent regulation of the DNA-dependent protein kinase.Cell Prolif. 32 (4): 239-48.
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