|Application ||WB, IHC-P, IP, GS|
|Calculated MW||469089 Da|
|Purification||Antisera to DNA-PKcs were raised by repeated immunisation of rabbit with highly purified antigen. Whole serum is supplied.|
|Immunogen||Human DNA-PKcs fragment.|
|Shelf Life||18 months from date of despatch.|
|Other Names||DNA-dependent protein kinase catalytic subunit, DNA-PK catalytic subunit, DNA-PKcs, 220.127.116.11, DNPK1, p460, PRKDC, HYRC, HYRC1|
|Target/Specificity||Rabbit anti-DNA PKcs antibody recognizes the human DNA-dependent protein kinase catalytic subunit, also known as DNA-PKcs, DNPK1 or p460. DNA PKcs is a 4128 amino acid ~460 kDa nuclear protein encoded by the PRKDC gene, acting as a molecular sensor for DNA damage. Mutation of the PRKDC gene can result in Immunodeficiency 26 with or without neurologic abnormalities (IMD26) (van der Burg et al.2009).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-DNA PKcs Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Finnie, N.J. et al. (1995) DNA-dependent protein kinase activity is absent in xrs-6 cells: implications for site-specific recombination and DNA double-strand break repair.Proc Natl Acad Sci U S A. 92 (1): 320-4. 2. Jayaram, S. et al. (2008) E1B 55k-independent dissociation of the DNA ligase IV/XRCC4 complex by E4 34k during adenovirus infection. Virology.382: 163-70. 3. Hoek, M. et al. (2011) An analysis of CAF-1-interacting proteins reveals dynamic and direct interactions with the KU complex and 14-3-3 proteins.J Biol Chem. 286 (12): 10876-87. 4. Frasca, D. et al. (2001) Role of DNA-dependent protein kinase in recognition of radiation-induced DNA damage in human peripheral blood mononuclear cells.Int Immunol. 13: 791-7. 5. Jayaram, S. et al. (2008) Loss of DNA ligase IV prevents recognition of DNA by double-strand break repair proteins XRCC4 and XLF.Nucleic Acids Res. 36: 5773-86. 6. Koike, M. et al. (1999) Differential subcellular localization of DNA-dependent protein kinase components Ku and DNA-PKcs during mitosis.J Cell Sci. 112: 4031-9. 7. Yee, M.C. et al. (2005) A cell-permeable, activity-based probe for protein and lipid kinases.J Biol Chem. 280: 29053-9. 8. Zhao, H.J. et al. (2000) DNA-dependent protein kinase activity correlates with Ku70 expression and radiation sensitivity in esophageal cancer cell lines.Clin Cancer Res. 6: 1073-8. 9. Sirzen, F. et al. (1999) DNA-dependent protein kinase content and activity in lung carcinoma cell lines: correlation with intrinsic radiosensitivity.Eur J Cancer. 35: 111-6. 10. Frasca, D. et al. (2002) The DNA repair protein ku is involved in gp130-mediated signal transduction events in PBMC from young but not from elderly subjects.Exp Gerontol. 37: 321-8. 11. Scarpaci, S. et al. (2003) DNA damage recognition and repair capacities in human naïve and memory T cells from peripheral blood of young and elderly subjects.Mech Ageing Dev. 124: 517-24. 12. Kamachi, M. et al. (2002) Human autoimmune sera as molecular probes for the identification of an autoantigen kinase signaling pathway.J Exp Med. 196: 1213-25. 13. Gaymes, T.J. et al. (2006) Histone deacetylase inhibitors (HDI) cause DNA damage in leukemia cells: a mechanism for leukemia-specific HDI-dependent apoptosis?Mol Cancer Res. 4 (8): 563-73. 14. Koike, M. et al. (1999) Differential subcellular localization of DNA-dependent protein kinase components Ku and DNA-PKcs during mitosis.J Cell Sci. 112 ( Pt 22): 4031-9. 15. Nilsson, A. et al. (1999) Cell cycle-dependent regulation of the DNA-dependent protein kinase.Cell Prolif. 32 (4): 239-48.
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