|Application ||IHC-P, IHC-F|
|Calculated MW||28385 Da|
|Purification||Antisera to Dynorphin A 1-17 were raised by repeated immunisation of rabbits with highly purified antigen. Whole serum is supplied - diluted.|
|Immunogen||Dynorphin A 1-17: Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln (porcine)|
|Shelf Life||18 months from date of despatch.|
|Other Names||Proenkephalin-B, Beta-neoendorphin-dynorphin, Preprodynorphin, Alpha-neoendorphin, Beta-neoendorphin, Big dynorphin, Big Dyn, Dynorphin A(1-17), Dyn-A17, Dynorphin A, Dynorphin A(1-13), Dynorphin A(1-8), Leu-enkephalin, Rimorphin, Dynorphin B, Dyn-B, Dynorphin B(1-13), Leumorphin, Dynorphin B-29, PDYN|
|Target/Specificity||Rabbit anti-Human dynorphin A antibody recognizes the opioid peptide dynorphin A (Fischli et al. 1982), a cleavage product of big dynorphin, itself derived from the precursor pro-encephalin-B, also known as preprodynorphin. Rabbit anti-human dynorphin A antibody binds to both dynorphin A 1-17 and 1-8 but not with other big dynorphin cleavage products such as dynorphin B, also known as rimorphin. Dynorphin A immunoreactive cells are found in the paraventricular and supraoptic nucleus and in hippocampal mossy fibers of rat brain following kainic acid administration and colchicine induced neurotoxicity (McGinty et al. 1983). Dynorphins play a role in multiple physiologic processes including pain perception (Wanget al.2001)and responses to stress (Ráczet al.2013). They mimic and compete with a number of opiate drugs(Chavkin 2013). Dysregulation of dynorphin A expression has been linked to progression of neurodegenerative conditions such as Altzheimer’s disease (Yakovlevaet al.2007) and appears to play a significant role in the pathobiology of epilepsy (Loackeret al.2007).Rabbit anti-human dynorphin antibody has been used successfully for the immunohistochemical demonstration of ‘mossy fibre’ sprouting in hippocampal sclerosis (Thomet al.2009) and in a virally induced disease model in the rat (Solbriget al.2006).|
|Preservative & Stabilisers||0.09% Sodium Azide; 0.1% Bovine Serum Albumin;|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Dynorphin A (aa1-17) Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Khachaturinan, H. et al. (1985) Pro-Dynorphin peptide immunocytochemistry in rhesus monkey brain.Peptides 6 (Suppl.2): 155-166. 2. Sherman, T.G. et al. (1988) The regulation of hypothalamic magnocellular neuropeptides and their mRNAs in the Brattleboro rat: Coordinate responses to further osmotic challenge.J. Neurosci. 8: 3785 - 3796. 3. Zardetto-Smith, A.M.et al. (1988) Lateral Hypothalamic Dynorphinergic Efferents to the Amygdala and Brainstem in the Rat.Peptides 9: 1121-1127. 4. Beck, H. et al. (2000) Synaptic plasticity in the human dentate gyrus.J. Neurosci. 20 (18): 7080-7086. 5. Thom, M. et al. (2009) Bilateral reorganization of the dentate gyrus in hippocampal sclerosis: a postmortem study.Neurology. 73: 1033-40. 6. Solbrig, M.V. et al. (2006) Kappa opioid control of seizures produced by a virus in an animal model.Brain. 129: 642-54. 7. Jeub, M. et al. (1999) Loss of dynorphin-mediated inhibition of voltage-dependent Ca2+ currents in hippocampal granule cells isolated from epilepsy patients is associated with mossy fiber sprouting.Neuroscience. 94: 465-71. 8. Beck, H. et al. (2000) Synaptic plasticity in the human dentate gyrus.J Neurosci. 20: 7080-6. 9. Knapp, P.E. et al. (2001) Endogenous opioids and oligodendroglial function: possible autocrine/paracrine effects on cell survival and development.Glia. 35: 156-65. 10. Dougherty, K.J. et al. (2009) Phenotypic Diversity and Expression of GABAergic Inhibitory Interneurons During Postnatal Development in Lumbar Spinal Cord of GAD67-GFP Mice.Neuroscience. 163: 909-19.
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