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Anti-Histone H4 (Ac8) Antibody (Serum)

  • WB - Anti-Histone H4 (Ac8) Antibody (Serum) ABD11299
    Published customer image:Rabbit anti Histone H4 antibody used for the evaluation of acetylated histone expression in yeast cell lysates by western blotting.Image caption:Eaf1p Was a Subunit of the NuA4 HAT (A) Eaf1-HA associated with NuA4 subunits. Immunoblots of analytical-scale TAP purifications are shown. The captured TAP-tagged protein is indicated above the gels, and the protein that was tested for association is indicated at the right. (B) Strains lacking EAF1 have defects in histone H4 acetylation. Whole cell extracts from mutant strains indicated on the top were tested for global histone acetylation using antibodies directed against different forms of acetylated H4 and H2A as indicated on the right.From: Kobor MS, Venkatasubrahmanyam S, Meneghini MD, Gin JW, Jennings JL, Link AJ, et al. A Protein Complex Containing the Conserved Swi2/Snf2-Related ATPase Swr1p Deposits Histone Variant H2A.Z into Euchromatin.PLoS Biol 2: e131.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P62805
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 11367 Da
Additional Information
Other Species D,Y,Mammalian,Amphibia,Plants
Purification Antisera to acetylated histone H4 were raised by repeated immunisation of rabbits with highly purified antigen.
Immunogen Ovalbumin-conjugated peptide: NSGRGKGGAcKGLGKYCc.
Shelf Life 18 months from date of despatch.
Gene ID 121504;554313;8294;8359;8360;8361;8362;8363;8364;8365;8366;8367;8368;8370
Other Names Histone H4, HIST1H4A, H4/A, H4FA
Target/Specificity Rabbit anti-Histone 4(Ac8) antibody recognizes histone H4 when acetylated at lysine 8.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Histone H4 (Ac8) Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Turner, B. M. et al. (1989) Histone H4 acetylation in human cells. Frequency of acetylation at different sites defined by immunolabelling with site-specific antibodies.
FEBS Letters 253: 141-145. 2. Turner, B. M. et al. (1992) Histone H4 isoforms acetylated at specific lysine residues define individual chromosomes and chromatin domains in Drosophila polytene nuclei.
Cell 69: 375-384. 3. Belyaev, N. D. et al. (1996) Differential underacetylation of histones H2A, H3 and H4 on the inactive X chromosome in human female cells.
Hum. Genet. 97: 573-578. 4. Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 8: 1. 5. Chen, W.Y. et al. (2000) Molecular mechanism for silencing virally transduced genes involves histone deacetylation and chromatin condensation.
Proc Natl Acad Sci U SA. 97: 377-82. 6. Friis, R.M. et al. (2009) A glycolytic burst drives glucose induction of global histone acetylation by picNuA4 and SAGA.
Nucleic Acids Res. 37: 3969-80. 7. Ito, K. et al. (2000) Glucocorticoid receptor recruitment of histone deacetylase 2 inhibits interleukin-1beta-induced histone H4 acetylation on lysines 8 and 12.
Mol Cell Biol. 20: 6891-903. 8. Kobor, M.S. et al (2004) A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin.
PLoS Biol. 2: E131. 9. Lin, X. et al. (2001) Reversal of GSTP1 CpG island hypermethylation and reactivation of pi-class glutathione S-transferase (GSTP1) expression in human prostate cancer cells by treatment with procainamide.
Cancer Res. 61: 8611-6. 10. Nie, M. et al. (2003) Transcriptional regulation of cyclooxygenase 2 by bradykinin and interleukin-1beta in human airway smooth muscle cells: involvement of different promoter elements, transcription factors, and histone h4 acetylation.
Mol Cell Biol. 23: 9233-44. 11. Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 8: 1. 12. Torres-Machorro, A.L. et al. (2015) The Set3 Complex Antagonizes the MYST Acetyltransferase Esa1 in the DNA Damage Response.
Mol Cell Biol. 35 (21): 3714-25.

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Cat# ABD11299
(40 western blots)
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