|Application ||WB, IF, E, IP, CHIP|
|Calculated MW||11367 Da|
|Other Species||Mammalian,D,Y,Plants,Amphibia,H,Arabidopsis,M,Zf,Wheat,Asellus aquaticus|
|Purification||Antisera to acetylated histone H4 were raised by repeated immunisation of rabbits with highly purified antigen.|
|Immunogen||KLH-conjugated peptide acetylated at lysines 5,8,12,16: NSGRGAcKGGAcKGLGAcKGGAAcKRHYCC.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Histone H4, HIST1H4A, H4/A, H4FA|
|Target/Specificity||Rabbit anti-Histone H4 (Acetylated) antibody was raised against the tetra-acetylated form of histone H4 and recognizes all acetylated isoforms of histone H4.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Histone H4 (Acetylated) Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Turner, B.M. et al. (1989) Histone H4 acetylation in human cells. Frequency of acetylation at different sites defined by immunolabeling with site-specific antibodies.FEBS Lett. 253 (1-2): 141-5. 2. Turner, B.M. et al. (1992) Histone H4 isoforms acetylated at specific lysine residues define individual chromosomes and chromatin domains in Drosophila polytene nuclei.Cell. 69 (2): 375-84. 3. Belyaev, N. et al. (1996) Differential underacetylation of histones H2A, H3 and H4 on the inactive X chromosome in human female cells.Hum Genet. 97 (5): 573-8. 4. Perry, P. et al. (2004) A dynamic switch in the replication timing of key regulator genes in embryonic stem cells upon neural induction.Cell Cycle. 3 (12): 1645-50. 5. Nye, A.C. et al. (2002) Alteration of large-scale chromatin structure by estrogen receptor.Mol Cell Biol. 22: 3437-49. 6. Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.J Inflamm (Lond). 8: 1. 7. Tian, L. and Chen, Z.J. (2001) Blocking histone deacetylation in Arabidopsis induces pleiotropic effects on plant gene regulation and development.Proc Natl Acad Sci U S A. 98: 200-5. 8. Beck, H.C. et al. (2006) Quantitative proteomic analysis of post-translational modifications of human histones.Mol Cell Proteomics. 5: 1314-25. 9. Catley, M.C. et al. (2004) Inhibitors of protein kinase C (PKC) prevent activated transcription: role of events downstream of NF-kappaB DNA binding.J Biol Chem. 279: 18457-66. 10. Collas, P. et al. (1999) Active transgenes in zebrafish are enriched in acetylated histone H4 and dynamically associate with RNA Pol II and splicing complexes.J Cell Sci. 112: 1045-54. 11. Foy, R.L. et al. (2008) Role of Jade-1 in the histone acetyltransferase (HAT) HBO1 complex.J Biol Chem. 283: 28817-26. 12. Lin, X. et al. (2001) Reversal of GSTP1 CpG island hypermethylation and reactivation of pi-class glutathione S-transferase (GSTP1) expression in human prostate cancer cells by treatment with procainamide.Cancer Res. 61: 8611-6. 13. Santos, A.P. et al. (2002) The architecture of interphase chromosomes and gene positioning are altered by changes in DNA methylation and histone acetylation.J Cell Sci. 115: 4597-605. 14. Barzotti, R. et al. (2006) DNA methylation, histone H3 methylation, and histone H4 acetylation in the genome of a crustacean.Genome. 49: 87-90. 15. Vega-Flores, G. et al. (2014) The GABAergic septohippocampal pathway is directly involved in internal processes related to operant reward learning.Cereb Cortex. 24 (8): 2093-107.
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