|Application ||WB, E|
|Calculated MW||30717 Da|
|Purification||Antisera to ovine IL-1 beta were raised by repeated immunisation of rabbits with highly purified antigen.|
|Immunogen||Recombinant IL-1 beta.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Interleukin-1 beta, IL-1 beta, IL1B|
|Target/Specificity||Rabbit anti-Sheep interleukin 1β antibody recognizes ovine interleukin-1 beta (IL-1 beta). No cross-reactivity is seen with ovine IL-6, IL-8, MCP-1 or TNF alpha.Rabbit anti-Sheep interleukin 1β antibody neutralizes the bioactivity of ovine IL-1 beta. Removal of sodium azide is recommended prior to use in functional assays.|
|Preservative & Stabilisers||0.1% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Sheep Interleukin-1 beta Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Rothel, J.S. et al. (1997) Analysis of ovine IL-1 beta production in vivoandin vitro by enzyme immunoassay and immunohistochemistry.Vet. Immunol. Immunopathol. 57: 267-278. 2. Bannerman, D.D. et al.(2004)Escherichia coliandStaphylococcus aureus elicit differential innate immune responses following intramammary infection.Clin Diagn Lab Immunol. 11: 463-72. 3. Dernfalk, J. et al. (2007) The xMAP technique can be used for detection of the inflammatory cytokines IL-1beta, IL-6 and TNF-alpha in bovine samples.Vet Immunol Immunopathol. 118: 40-9. 4. Moyes, K.M. et al. (2009) Identification of potential markers in blood for the development of subclinical and clinical mastitis in dairy cattle at parturition and during early lactation.J Dairy Sci. 92: 5419-28. 5. Allen, J.S. et al. (2009) Plasmacytoid dendritic cells are proportionally expanded at diagnosis of type 1 diabetes and enhance islet autoantigen presentation to T-cells through immune complex capture.Diabetes. 2009 Jan;58(1):138-45. 6. Vordermeier, M. et al. (2012) Cytokine responses of Holstein and Sahiwal zebu derived monocytes after mycobacterial infection.Trop Anim Health Prod. 44: 651-5. 7. Xu, A. et al. (2015) The Ovine Fetal and Placental Inflammatory Response to Umbilical Cord Occlusions With Worsening Acidosis.Reprod Sci. pii: 1933719115580994. 8. Bagu, E.T. et al. (2010) Post-natal changes in testicular concentrations of interleukin-1 alpha and beta and interleukin-6 during sexual maturation in bulls.Reprod Domest Anim. 45 (2): 336-41. 9. Atik, A. et al. (2012) Long-term pulmonary effects of intrauterine exposure to endotoxin following preterm birth in sheep.Reprod Sci. 19 (12): 1352-64. 10. Silvestre, F.T. et al. (2011) Effects of differential supplementation of fatty acids during the peripartum and breeding periods of Holstein cows: II. Neutrophil fatty acids and function, and acute phase proteins.J Dairy Sci. 94 (5): 2285-301.
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