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Anti-Human DFF (C-Terminal) Antibody

Rabbit Anti-Human Polyclonal Antibody

  • ICC - Anti-Human DFF (C-Terminal) Antibody  ABD11307
    Immunocytochemical staining of HeLa cells with Rabbit anti Human DFF
  • WB - Anti-Human DFF (C-Terminal) Antibody  ABD11307
    Western blot analysis of whole cell lysates from HeLa (H), Jurkat (J), A431 (A) and K562 (K) cell lines probed with Rabbit anti Human DFF
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O00273
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 36522 Da
Additional Information
Purification Antisera to DFF were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
Immunogen Peptide from C-terminus of human DFF
Shelf Life 18 months from date of despatch.
Gene ID 1676
Other Names DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit, DFF-45, Inhibitor of CAD, ICAD, DFFA, DFF1, DFF45
Target/Specificity ABD11307 recognises the 45kD chain of a heterodimeric apoptosis related protein designated DFF (DNA fragmentation factor). DFF-45 is a substrate for Caspase-3, which cleaves the protein at two sites to generate factors that cause DNA fragmentation.ABD11307 recognises both intact DFF-45 and cleaved fragments at the C-terminus.
Preservative & Stabilisers 0.02% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human DFF (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Gabdoulkhakova, A. et al. (2007) High rate of mutation reporter gene inactivation during human T cell proliferation.
Immunogenetics. 59: 135-43.1. Liu, X. et al. (1997) DFF, a heterodimeric protein that functions downstream of Caspase-3 to trigger DNA fragmentation during apoptosis.
Cell 89: 175-184.

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Cat# ABD11307
(40 western blots)
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