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Anti-Human alpha Fetoprotein Antibody

Rabbit Anti-Human Polyclonal Antibody

  • E - Anti-Human alpha Fetoprotein Antibody  ABD11319
    Published customer image:Rabbit anti Human alpha Fetoprotein antibody used for the detection of alpha fetoprotein in dendritic cells by immunofluorescence.Image caption:Adenovirally-expressed AFP, but not eGFP-AFP, is secreted by transduced DC. a DC were transduced with AdVhAFP (left panel), AdVeGFP-AFP (middle panel), or AdVTyrosinase at MOI 2000 for 3 hr. After 48 hr, cells were fixed and stained for AFP (left and middle panels), actin (left and right panels), or Tyrosinase (right panel). Antibody staining of AdVeGFP-AFP-transduced cells with an anti-AFP antibody reveals that AFP and GFP colocalize, as expected. Representative images from three independent experiments are shown. Images were taken using a 63x objective. b DC from healthy donors (n?=?3) were transduced as above and cultured in DC media for 24, 48, or 72 hr. Supernatants were analyzed by AFP ELISA. Data are reported as the mean?±?SDFrom: Pardee AD, Yano H, Weinstein AM, Ponce AA, Ethridge AD, Normolle DP, Vujanovic L, Mizejewski GJ, Watkins SC, Butterfield LH. Route of antigen delivery impacts the immunostimulatory activity of dendritic cell-based vaccines for hepatocellular carcinoma.J Immunother Cancer. 2015 Jul 21;3:32.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P02771
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 68678 Da
Additional Information
Other Species Pig,Sh
Purification Antisera to human AFP were raised by repeated immunisation of rabbits with highly purified antigen
Immunogen Human Alpha-1-Fetoprotein (cord serum)
Shelf Life 18 months from date of despatch.
Gene ID 174
Other Names Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP
Target/Specificity Rabbit anti-Human alpha Fetoprotein antibody recognizes human alpha fetoprotein, and shows positive staining of parenchymal cells of fetal liver and neoplastic liver cells showing yolk sac differentiation in germ cell tumours.
Preservative & Stabilisers 0.1% Sodium Azide (NaN3); 1% Bovine Serum Albumin;
Storage Store at +4℃. DO NOT FREEZE.
PrecautionsAnti-Human alpha Fetoprotein Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Pardee, A.D. et al. (2015) Route of antigen delivery impacts the immunostimulatory activity of dendritic cell-based vaccines for hepatocellular carcinoma.
J Immunother Cancer. 3: 32.

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Cat# ABD11319
(40 western blots)
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