|Application ||WB, IHC-F, E|
|Calculated MW||137910 Da|
|Purification||Antisera to phosphorylated human HER2/neu were raised by repeated immunisation of rabbits with highly purified antigen.|
|Immunogen||Threonine phosphorylated peptide KIRKYTMRRLL from HER2/neu sequence.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Receptor tyrosine-protein kinase erbB-2, 188.8.131.52, Metastatic lymph node gene 19 protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, CD340, ERBB2, HER2, MLN19, NEU, NGL|
|Target/Specificity||This antibody recognises human HER2/neu, otherwise known as CD340 and c-ErbB-2, phosphorylated at threonine residue (amino acid 686). Some binding to unphosphorylated HER2/neu may be seen at higher concentrations, but this may be overcome by appropriate titration. HER2/neu is a proto-oncogene expressed at the cell surface of a range of tumour cells, which is often associated with poor prognosis, and an increased risk of metastasis, and is used in conjunction with antibodies recognising both Estrogen and Progesterone receptors, for the classification of breast cancer tumours, and to determine patient prognosis and course of treatment. Excessive HER2/neu is present in around 20% to 25% of invasive breast cancers, referred to as HER2-positive cancer, and can help determine whether drugs such as trastuzumab (Herceptin) or lapatinib (Tykerb), might be beneficial.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human HER2/neu (pThr686) Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Ouyang, X. et al. (1996) Human cancer cells exhibit protein kinase C-dependent c-erbB-2 transmodulation that correlates with phosphatase sensitivity and kinase activity. J. Biol. Chem. 271: 21768 - 21792.
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