|Application ||WB, IHC-P|
|Calculated MW||86943 Da|
|Purification||Antisera to APP were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.|
|Immunogen||Synthetic peptide corresponding to amino acids 737-751 of human APP.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Amyloid beta A4 protein, ABPP, APPI, APP, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II, N-APP, Soluble APP-alpha, S-APP-alpha, Soluble APP-beta, S-APP-beta, C99, Beta-amyloid protein 42, Beta-APP42, Beta-amyloid protein 40, Beta-APP40, C83, P3(42), P3(40), C80, Gamma-secretase C-terminal fragment 59, Amyloid intracellular domain 59, AICD-59, AID(59), Gamma-CTF(59), Gamma-secretase C-terminal fragment 57, Amyloid intracellular domain 57, AICD-57, AID(57), Gamma-CTF(57), Gamma-secretase C-terminal fragment 50, Amyloid intracellular domain 50, AICD-50, AID(50), Gamma-CTF(50), C31, APP, A4, AD1|
|Target/Specificity||Rabbit anti-Amyloid Precursor Protein antibody recognizes both intact Amyloid Precursor Protein (APP), and also the C99 fragment generated by Beta-secretase.The sequence recognised by this antibody corresponds to amino acids 85-99 of the C99 fragment. The C99 fragment itself is a substrate for gamma-secretase to generate the 4 kDa beta amyloid peptide, found in the brains of Alzheimer's disease patients. APP also inhibits Notch signaling through it’s interaction with NUMB (Roncaratiet al.2002). Rabbit anti-Amyloid Precursor Protein antibody has been used successfully for the detection of the ~14 kDa β C-terminal fragment of APP, producted as a result of cleavage by BACE1 using western blotting -in rat retinal lysates (Huanget al.2012).|
|Preservative & Stabilisers||0.02% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Amyloid Precursor Protein (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Yan, X.X. et al. (2007) beta-Secretase expression in normal and functionally deprived rat olfactory bulbs: inverse correlation with oxidative metabolic activity.J Comp Neurol. 501: 52-69. 2. Zhang, H. et al. (2011) IGF-1 reduces BACE-1 expression in PC12 cells via activation of PI3-K/Akt and MAPK/ERK1/2 signaling pathways.Neurochem Res. 36: 49-57. 3. Désiré, L. et al. (2005) RAC1 inhibition targets amyloid precursor protein processing by gamma-secretase and decreases Abeta production in vitroandin vivo.J Biol Chem. 280: 37516-25. 4. Huang JF et al. (2012) Timosaponin-BII inhibits the up-regulation of BACE1 induced by ferric chloride in rat retina.BMC Complement Altern Med. 12: 189. 5. Xiong, K. et al. (2007) Mitochondrial respiratory inhibition and oxidative stress elevate beta-secretase (BACE1) proteins and activity in vivo in the rat retina.Exp Brain Res. 181: 435-46. 6. Zhang, X.M. et al. (2010) Functional deprivation promotes amyloid plaque pathogenesis in Tg2576 mouse olfactory bulb and piriform cortex.Eur J Neurosci. 31: 710-21. 7. Cai, Y. et al. (2010) β-Secretase-1 elevation in aged monkey and Alzheimer's disease human cerebral cortex occurs around the vasculature in partnership with multisystem axon terminal pathogenesis and β-amyloid accumulation.Eur J Neurosci. 32: 1223-38. 8. Marcade, M. et al. (2008) Etazolate, a neuroprotective drug linking GABA(A) receptor pharmacology to amyloid precursor protein processing.J Neurochem. 106: 392-404. 9. Cai, Y. et al. (2012) BACE1 elevation is involved in amyloid plaque development in the triple transgenic model of Alzheimer's disease: differential Aβ antibody labeling of early-onset axon terminal pathology.Neurotox Res. 21: 160-74. 10. Zhang, H. et al. (2015) Hydrogen sulfide-induced processing of the amyloid precursor protein in SH-SY5Y human neuroblastoma cells involves the PI3-K/Akt signaling pathway.Cell Mol Neurobiol. 35 (2): 265-72. 11. Xue, Z.Q. et al. (2015) Non-neuronal and neuronal BACE1 elevation in association with angiopathic and leptomeningeal ß-amyloid deposition in the human brain.BMC Neurol. 15: 71.1. Ponte, P. et al. (1988) A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors.Nature 331: 525-527.2. Selkoe, D.J. (1994) Cell biology of the amyloid beta protein precursor and the mechanism of Alzheimer's disease.Ann. Rev. Cell. Biol. 10: 373-403.
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