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Anti-Smac/Diablo (C-Terminal) Antibody

Rabbit Anti-Mouse Polyclonal Antibody

  • WB - Anti-Smac/Diablo (C-Terminal) Antibody  ABD11370
    Western blot analysis of Smac/DIABLO in mouse heart tissue lysate in the absence (A) or presence (B) of blocking peptide and in rat heart tissue lysate probed with Rabbit anti Smac/Diablo antibody
  • IHC - Anti-Smac/Diablo (C-Terminal) Antibody  ABD11370
    Imunohistochemical staining of mouse spleen with Rabbit anti Mouse Smac/DIABLO
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9JIQ3
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 26820 Da
Additional Information
Other Species Rat,H
Purification Antisera to mouse Smac/DIABLO were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen Synthetic peptide corresponding to amino acids 222-237 of mouse Smac/DIABLO.
Shelf Life 18 months from date of despatch.
Gene ID 66593
Other Names Diablo homolog, mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase, Smac, Diablo, Smac
Target/Specificity Rabbit anti-Smac/Diablo antibody recognizes Diablo homolog, mitochondrial, also known as Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase, Diablo or Smac. Smac is a 237 amino acid ~30 kDa mitochondrial protein bearing a 53 amino acid transit peptide at its N-terminus. Rabbit anti-Smac/Diablo antibody binds to the C-terminal region of murine Smac/DIABLO. Inhibitors of apoptosis proteins (IAP's) block cell death by caspase inhibition. X-linked inhibitor of apoptosis (XIAP) is a member of the IAP family. Smac/DIABLO is expressed in a variety of tissues and has been identified as a negative regulator of XIAP (Verhagen 2000).
Preservative & Stabilisers 0.02% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Smac/Diablo (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Verhagen, A. M. et al (2001) Inhibitor of apoptosis proteins and their relatives; IAP's and other BIRPS's. Genome Biol.
2(7): Reviews 3009.2. Wu, G. et al (2000) Structural basis of IAP recognition by Smac/DIABLO.
Nature. 408: 1008-12

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Cat# ABD11370
(40 western blots)
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