|Calculated MW||74642 Da|
|Purification||Antisera to human MELK kinase were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.|
|Immunogen||Peptide sequence KRLVEDILSSCKV corresponding to the C-terminus (CT) of MELK.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Maternal embryonic leucine zipper kinase, hMELK, 22.214.171.124, Protein kinase Eg3, pEg3 kinase, Protein kinase PK38, hPK38, Tyrosine-protein kinase MELK, 126.96.36.199, MELK, KIAA0175|
|Target/Specificity||ABD11386 is specific for human maternal embryonic leucine zipper kinase (MELK), also known as KIAA0175. MELK is a member of the AMPK family, which has several consensus glycosylation sites.ABD11386 consistently detects a band of approximately 120-150kD in Jurkat and Hela cell lysates, which is larger than the predicted size of 72kD. The activity of the antibody has been confirmed against recombinant MELK protein by western blotting.|
|Preservative & Stabilisers||0.02% Sodium Azide; 0.5% Bovine Serum Albumin;|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human MELK (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Nagase, T. et al. (1996) Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1.DNA Res 3: 17 - 24 2. Niesler, C.U. et al. (2007) The changing AMPK expression profile in differentiating mouse skeletal muscle myoblast cells helps confer increasing resistance to apoptosis.Exp Physiol. 92: 207-17.
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