|Application ||WB, IHC-F, IF, IP|
|Calculated MW||25135 Da|
|Purification||Antisera to GAP43 were raised by repeated immunisations of rabbits with highly purified antigen.|
|Immunogen||Purified GAP43 from cat brain.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Neuromodulin, Axonal membrane protein GAP-43, Growth-associated protein 43, GAP43|
|Target/Specificity||Rabbit anti-GAP43 antibody recognises the protein designated GAP43, a neuronal protein that is expressed at elevated levels during development and also during regenerative axon growth.GAP43 (also known as neuromodulin, pp46, B-50, Protein F1 and calmodulin-binding protein P-57) is a protein of approximately 25kD that migrates on SDS-PAGE gels with an apparent size of 40-50kD. The aberrant migration is due to the unusually low abundance of hydrophobic amino acid residues. GAP43 is found tightly associated with neuronal membranes due to palmitoylation. The solubilised protein binds calmodulin, although this is reduced following protein kinase C phosphorylation of GAP43.|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-GAP43 Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. McIntosh, H. et al. (1989) A GAP-43-like protein in cat visual cortex.Vis Neurosci. 2 (6): 583-91. 2. Wang, H. et al. (2009) Neuroprotective effect of recombinant human erythropoietin on optic nerve injury in rats.Chin Med J 122: 2008-12. 3. Zhang, H. et al. (2010) Alteration of parasympathetic/sympathetic ratio in the infarcted myocardium after Schwann cell transplantation modified electrophysiological function of heart: a novel antiarrhythmic therapy.Circulation. 122 (11 Suppl): S193-200. 4. Parikh, P. et al. (2011) Regeneration of axons in injured spinal cord by activation of bone morphogenetic protein/Smad1 signaling pathway in adult neurons.Proc Natl Acad Sci U S A. 108: E99-107. 5. Turner, J.H. et al. (2010) Reversible loss of neuronal marker protein expression in a transgenic mouse model for sinusitis-associated olfactory dysfunction.Am J Rhinol Allergy. 24: 192-6. 6. Su, Y. et al. (2008) Axonal regeneration after optic nerve crush in Nogo-A/B/C knockout mice.Mol Vis. 14: 268-73. 7. Pao PC et al. (2011) A novel RING finger protein, Znf179, modulates cell cycle exit and neuronal differentiation of P19 embryonal carcinoma cells.Cell Death Differ. 18 (11): 1791-804. 8. Cheng, H.T. et al. (2013) Increased Axonal Regeneration and Swellings in Intraepidermal Nerve Fibers Characterize Painful Phenotypes of Diabetic Neuropathy.J Pain. pii: S1526-5900(13)00910-3.
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