|Application ||IHC-F, IF|
|Calculated MW||131141 Da|
|Purification||Antisera to mannosidase II were raised by repeated immunisations of rabbits with highly purified antigen.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Alpha-mannosidase 2, 126.96.36.199, Golgi alpha-mannosidase II, AMan II, Man II, Mannosidase alpha class 2A member 1, Mannosyl-oligosaccharide 1, 3-1, 6-alpha-mannosidase, MAN2A1, MANA2|
|Target/Specificity||ABD11400 recognises mannosidase II, a 135kD protein located on the luminal side of golgi membranes.The antibody is useful as a marker for golgi apparatus.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Mannosidase II Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Herbert, S.P. et al. (2005) Cytosolic phospholipase A2-alpha mediates endothelial cell proliferation and is inactivated by association with the Golgi apparatus. Mol Biol Cell. 16: 3800-9. 2. Desai, P. et al. (2008) Localization of herpes simplex virus type 1 UL37 in the Golgi complex requires UL36 but not capsid structures. Epub 2008 Sep 10. PubMed PMID: J Virol. 82:11354-61. 3. Tangemo, C. et al. (2011) A novel laser nanosurgery approach supports de novo Golgi biogenesis in mammalian cells.J Cell Sci. 124: 978-87. 4. Herbert, S.P. ert al. (2007) The confluence-dependent interaction of cytosolic phospholipase A2-alpha with annexin A1 regulates endothelial cell prostaglandin E2 generation.J Biol Chem. 282: 34468-78. 5. Rybakin, V. et al. (2008) Molecular mechanism underlying the association of Coronin-7 with Golgi membranes.Cell Mol Life Sci. 65: 2419-30.
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