|Application ||WB, IP|
|Calculated MW||15145 Da|
|Purification||Antisera to human H2AX were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||Synthetic peptide corresponding to a region within the C-terminus of human H2AX (locus link ID 3014)|
|Shelf Life||18 months from date of despatch.|
|Other Names||Histone H2AX, H2a/x, Histone H2A.X, H2AFX, H2AX|
|Target/Specificity||Rabbit anti-Human Histone 2AX antibody recognizes human histone 2AX (H2AX), a 143 amino acid, ~15kDa member of the histone H2A family differing from other members in having a unique carboxy-terminal region containing a conserved serine, four bases from the carboxy terminus (Redon 2002) which becomes rapidly phosphorylated following DNA double stranded breaks (Rogakouet al.1998).H2AX is involved in DNA repair and the maintenance of genomic stability, although its precise role is still to be defined. H2AX is phosphorylated soon after the appearance of double-stranded breaks, and has been implicated both in homologous recombination and non-homologous end joining DNA repair pathways. H2AX is thought to have a critical function in the recruitment of DNA repair factors and DNA damage-signaling proteins (Scully and Xie 2013). Phoshorylation of H2AX is linked to DNA fragmentation during apoptosis (Rogakuet al.2000).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃. DO NOT FREEZE.|
|Precautions||Anti-Human Histone H2AX Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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