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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   Anti-Human RAD17 (pSer645) Antibody    

Anti-Human RAD17 (pSer645) Antibody

Rabbit Anti-Human Polyclonal Antibody

  • WB - Anti-Human RAD17 (pSer645) Antibody  ABD11444
    Detection of human phospho-Rad17 in whole cell lysates of HeLa cells either treated with 10Gy ionizing radiation or untreated by Western blotting using Rabbit anti Human phospho-Rad17
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75943
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 77055 Da
Additional Information
Purification Antisera to human phosphorylated RAD17 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen Phosphorylated synthetic peptide corresponding to a region within human RAD17 (locus link ID 5884) around serine 645.
Shelf Life 18 months from date of despatch.
Gene ID 5884
Other Names Cell cycle checkpoint protein RAD17, hRad17, RF-C/activator 1 homolog, RAD17, R24L
Target/Specificity Rabbit anti-Human RAD17 (pSer645) antibody recognizes human RAD17 which is phosphorylated at Ser645.RAD17 is a cell cycle checkpoint protein required for activation of checkpoint signaling pathways in response to stalled replication forks and DNA damage. RAD17 is phosphorylated on Ser635 and Ser645 is response to DNA damage and replication inhibitors. Phosphorylation at these residues is also reported in undamaged cycling cells during S and G2/M (Postet al.2001).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human RAD17 (pSer645) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Post, S. et al. (2001) Phosphorylation of serines 635 and 645 of human Rad17 is cell cycle regulated and is required for G1/S checkpoint activation in response to DNA damage.
Proc. Natl. Acad. Sci. USA. 6:13102-13107.

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Cat# ABD11444
(40 western blots)
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