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Anti-Human Sp1 Antibody

Rabbit Anti-Human Polyclonal Antibody

  • WB - Anti-Human Sp1 Antibody  ABD11447
    Western blot analysis of HeLa nuclear extract probed with Rabbit anti Human Sp1 followed by SHEEP ANTI RABBIT IgG:HRP
  • WB - Anti-Human Sp1 Antibody  ABD11447
    Detection of human Sp1 in whole cell lysates of HEK293T cells transfected with a human Sp1 construct (T) or mock transfected (E) using Rabbit anti Human Sp1 and other anti Sp1 antibodies for immunoprecipitation at 3.75 mcg/mg lysate. Subsequently, membranes were probed with Rabbit anti Human Sp1 antibody 0.04mg/ml for detection by Western Blotting
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08047
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 80693 Da
Additional Information
Purification Antisera to human Sp1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen Synthetic peptide corresponding to residues between 750 and 785 of Sp1
Shelf Life 18 months from date of despatch.
Gene ID 6667
Other Names Transcription factor Sp1, SP1, TSFP1
Target/Specificity Rabbit anti-Human Sp1 antibody recognizes human Transcription factor Sp1 (Sp1), also known as TSFP1. Sp1 is a 785 amino acid, ~95kDa member of the Sp1 C2H2-type zinc-finger protein family. containing 3 C2H2-type zinc finger motifs. Sp1 is a transcription regulator, involved in the regulation of multiple genes. Sp1 has been implicated in a range of cellular processes including cell cycle regulation, maintenance of gene activation during development (Marinet al.1997), regulation of nucleic acid metabolism and preservation of chromatin structure at gene loci. The transcription factor Sp1 binds to GC-rich motifs within DNA, and has been shown to interact with other tissue-specific transcription factors to modulate the activities of a variety of promoters (Hölleret al.1988). Sp1 is ubiquitously expressed in cell nuclei, although the level of expression may vary between cell types (Resendes and Rosmarin 2004).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Sp1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Stark, M. et al. (2006) Loss of Sp1 function via inhibitory phosphorylation in antifolate-resistant human leukemia cells with down-regulation of the reduced folate carrier.
Blood. 107:708-715.

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Cat# ABD11447
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