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Anti-Human Histone H2AX Antibody

Rabbit Anti-Human Polyclonal Antibody

  • WB - Anti-Human Histone H2AX Antibody  ABD11458
    Detection of human and mouse histone H2AX in nuclear extracts from human 293T and wild type (+/+) or H2AX knockout (-/-) mouse embryonic fibroblasts by Western blotting using Rabbit anti H2AX
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P16104
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 15145 Da
Additional Information
Purification Antisera to human H2AX were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen Synthetic peptide corresponding to a region within the C-terminus of human H2AX.
Shelf Life 18 months from date of despatch.
Gene ID 3014
Other Names Histone H2AX, H2a/x, Histone H2A.X, H2AFX, H2AX
Target/Specificity Rabbit anti-Human Histone H2AX antibody recognizes human histone H2AX.H2AX comprises approximately 2-25% of the histone H2A pool in mammalian cells. The extended C-terminal tail of H2AX contains a serine (Ser139) embedded in an conserved SQE motif, which is rapidly phosphorylated at sites of double strand breaks and stalled replication forks. Phosphorylated H2AX may serve to recruit other repair proteins to the site of DNA damage.H2AX has been implicated in both homologous recombination and nonhomologous end joining DNA repair pathways.
Preservative & Stabilisers 0.1% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Histone H2AX Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD11458
(40 western blots)
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