|Calculated MW||54115 Da|
|Purification||Antisera to phosphorylated rat CaMK II were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG was prepared by affinity chromatography.|
|Immunogen||Synthetic phosphopeptide corresponding to an amino acid sequence within CaMK II which includes phosphorylated Thr286.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha, 220.127.116.11, Camk2a|
|Target/Specificity||Rabbit anti-phosphorylated CaMK II (pThr286) antibody recognizes rat calcium/calmodulin kinase II (CaMK II) alpha and CaMK II beta, when phosphorylated at Thr286.CaMKII is a multi-functional calcium and calmodulin-dependent kinase which has an important role in cell signalling and becomes autonomously active when phosphorylated at Thr286. Autophosphorylation of the α subunit at threonine 286 confers calmodulin independent kinase activity (Theilet al.1988).|
|Preservative & Stabilisers||0.09% Sodium Azide; 0.01% Bovine Serum Albumin; 50% Glycerol|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-CaMK II (pThr286) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Lin, C.R. et al. (1987) Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase.Proc Natl Acad Sci U S A. 84 (16): 5962-6.
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