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Anti-CDC2 (pTyr15) Antibody

     
  • WB - Anti-CDC2 (pTyr15) Antibody  ABD11468
    Western blot of human T47D cells showing phosphospecific immunolabeling of the ~34 kDa cdc 2 protein phosphoylated at Tyr15. In lane 2, cells were treated with EGF leading to dephosphorylation of Tyr15
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P06493
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 34095 Da
Additional Information
Other Species M,X,H
Purification Antisera to phosphorylated CDC2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Immunogen Synthetic phosphopeptide corresponding to an amino acid sequence within CDC2 which includes phosphorylated Tyr15.
Shelf Life 12 months from date of reconstitution.
Gene ID 983
Other Names Cyclin-dependent kinase 1, CDK1, 2.7.11.22, 2.7.11.23, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase, CDK1, CDC2, CDC28A, CDKN1, P34CDC2
Target/Specificity Rabbit anti-phosphorylated CDC2 antibody recognizes Cyclin-dependent kinase 1, also known as Cell division control protein 2 homolog, CDC2, CDK1 or p34 protein kinase. Rabbit anti-phosphorylated CDC2 antibody recognizes CDC2 only when phosphorylated at Tyr15.CDC2 is a serine kinase which plays an important role in the regulation of the cell cycle, mediating progression from G2 to M phase. Phosphorylation of tyrosine 15 results in inactivation of CDC2.
Preservative & Stabilisers 0.09% Sodium Azide; 0.01% Bovine Serum Albumin; 50% Glycerol
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-CDC2 (pTyr15) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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References

1. Fletcher, L. et al. (2002) Abolishment of the Tyr-15 inhibitory phosphorylation site on cdc2 reduces the radiation-induced G2 delay, revealing a potential checkpoint in early mitosis.
Cancer Res. 62: 241-250.

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Cat# ABD11468
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