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Anti-CREB (pSer133) Antibody

Rabbit Anti-Rat Polyclonal Antibody

  • WB - Anti-CREB (pSer133) Antibody  ABD11469
    Western blot of a forskolin-stimulated rat hippocampal slice showing phosphospecific immunolabeling of the ~45 kDa CREB protein phosphphorylated at Ser133
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P15337
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 36633 Da
Additional Information
Purification Antisera to phosphorylated rat CREB were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Immunogen Synthetic phosphopeptide corresponding to an amino acid sequence within CREB which includes phosphorylated Ser133.
Shelf Life 12 months from date of reconstitution.
Gene ID 81646
Other Names Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, Creb1, Creb-1
Target/Specificity Rabbit anti-phosphorylated CREB antibody recognizes rat cyclic AMP response element binding protein 1, also known as CREB or CREB-1, when phosphorylated at serine 133.CREB is a member of the leucine zipper family of transcription factors, which is involved in a wide range of intracellular signalling processes.Phosphorylation of serine 133 has been demonstrated by several protein kinases, including protein kinase A (PKA), mitogen activated protein kinase (MAPK) and Ca2+ /calmodulin-dependent protein kinase (CaMK). This phophorylation is required for CREB mediated transcription.
Preservative & Stabilisers 0.09% Sodium Azide; 0.01% Bovine Serum Albumin; 50% Glycerol
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-CREB (pSer133) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Mayr, B.M. et al. (2001) Distinct effects of cAMP and mitogenic signals on CREB-binding protein recruitment impart specificity to target gene activation via CREB.
Proc Natl Acad Sci U S A. 98 (19): 10936-41.

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Cat# ABD11469
(40 western blots)
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