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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Darpp-32 (pThr34) Antibody    

Anti-Darpp-32 (pThr34) Antibody

Rabbit Anti-Rat Polyclonal Antibody

  • WB - Anti-Darpp-32 (pThr34) Antibody  ABD11470
    Western blot of rat caudate showing phosphospecific immunolabeling of the ~32 kDa DARPP-32 protein phosphorylated at Thr34
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q6J4I0
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 22913 Da
Additional Information
Purification Antisera to phosphorylated rat DARPP-32 were raised by repeated immunisations of rabbits with highly purified antigen.
Immunogen Synthetic phosphopeptide corresponding to an amino acid sequence within DARPP-32 which includes phosphorylated Thr34.
Shelf Life 12 months from date of reconstitution.
Gene ID 360616
Other Names Protein phosphatase 1 regulatory subunit 1B, DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, Ppp1r1b
Target/Specificity Rabbit anti-Rat DARP-32 (pThr34) antibody recognizes rat DARPP-32, when phosphorylated at threonine 34. DARPP-32 is also known as Protein phosphatase 1 regulatory subunit 1B or Dopamine- and cAMP-regulated neuronal phosphoprotein. DARPP-32 is a 205 amino acid ~32 kDa member of the protein phosphatase inhibitor 1 family.DARPP-32 is principally expressed in striatal medium spiny neurons, and plays a critical role in the regulation of dopaminergic neurotransmission.DARPP-32 can act either as a phosphatase inhibitor or as a kinase inhibitor, depending on its relative state of phosphorylation . Phosphorylation at threonine 34 converts DARPP-32 into an inhibitor of protein phosphatase-1 (PP-1) whilst phosphorylation at threonine 75 switches the protein to an inhibitor of protein kinase A (PKA) . G-protein coupled receptor 6 deficiency in a mouse model of Parkinsons disease leads to an increase in DARPP-32 (pThr34) in striatopalladial neurons with a concommitent increase in locomotor activity and reduced abnormal movements in the mouse dyskinesia model of Parkinson's disease, thus suggesting treatment other than dopamine replacement for the condition (Oeklet al.2014).
Preservative & Stabilisers 0.09% Sodium Azide; 0.1% Bovine Serum Albumin; 50% Glycerol
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Darpp-32 (pThr34) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Xiao, M.F. et al. (2009) Neural cell adhesion molecule modulates dopaminergic signaling and behavior by regulating dopamine D2 receptor internalization.
J Neurosci. 29: 14752-63. 2. Oeckl, P. et al. (2014) G-protein coupled receptor 6 deficiency alters striatal dopamine and cAMP concentrations and reduces dyskinesia in a mouse model of Parkinson's disease
Exp Neurol. 257C: 1-9.

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Cat# ABD11470
(40 western blots)
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