|Calculated MW||22913 Da|
|Purification||Antisera to phosphorylated rat DARPP-32 were raised by repeated immunisations of rabbits with highly purified antigen.|
|Immunogen||Synthetic phosphopeptide corresponding to an amino acid sequence within DARPP-32 which includes phosphorylated Thr34.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Protein phosphatase 1 regulatory subunit 1B, DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, Ppp1r1b|
|Target/Specificity||Rabbit anti-Rat DARP-32 (pThr34) antibody recognizes rat DARPP-32, when phosphorylated at threonine 34. DARPP-32 is also known as Protein phosphatase 1 regulatory subunit 1B or Dopamine- and cAMP-regulated neuronal phosphoprotein. DARPP-32 is a 205 amino acid ~32 kDa member of the protein phosphatase inhibitor 1 family.DARPP-32 is principally expressed in striatal medium spiny neurons, and plays a critical role in the regulation of dopaminergic neurotransmission.DARPP-32 can act either as a phosphatase inhibitor or as a kinase inhibitor, depending on its relative state of phosphorylation . Phosphorylation at threonine 34 converts DARPP-32 into an inhibitor of protein phosphatase-1 (PP-1) whilst phosphorylation at threonine 75 switches the protein to an inhibitor of protein kinase A (PKA) . G-protein coupled receptor 6 deficiency in a mouse model of Parkinsons disease leads to an increase in DARPP-32 (pThr34) in striatopalladial neurons with a concommitent increase in locomotor activity and reduced abnormal movements in the mouse dyskinesia model of Parkinson's disease, thus suggesting treatment other than dopamine replacement for the condition (Oeklet al.2014).|
|Preservative & Stabilisers||0.09% Sodium Azide; 0.1% Bovine Serum Albumin; 50% Glycerol|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Darpp-32 (pThr34) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Xiao, M.F. et al. (2009) Neural cell adhesion molecule modulates dopaminergic signaling and behavior by regulating dopamine D2 receptor internalization.J Neurosci. 29: 14752-63. 2. Oeckl, P. et al. (2014) G-protein coupled receptor 6 deficiency alters striatal dopamine and cAMP concentrations and reduces dyskinesia in a mouse model of Parkinson's diseaseExp Neurol. 257C: 1-9.
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