|Calculated MW||22913 Da|
|Purification||Antisera to phosphorylated rat DARPP-32 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||Synthetic phosphopeptide corresponding to an amino acid sequence within DARPP-32 which includes phosphorylated Thr75.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Protein phosphatase 1 regulatory subunit 1B, DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, Ppp1r1b|
|Target/Specificity||Rabbit anti-Rat DARPP-32 (pThr75) antibody recognises rat DARPP-32, when phosphorylated at threonine 75. DARPP-32 is a 205 amino acid ~32 kDa member of the protein phosphatase inhibitor 1 family. Originally discovered as a substrate for cAMP-dependent protein kinase which modulates cell signalling in neurons. DARPP-32 is converted into an inhibitor of protein phosphatase-1 when phosphorylated by protein kinase A at threonine 34, as a result of dopaminergic receptor stimulation (Bibbet al.1999). However this phosphorylation is reversed on stimulation of N-methyl-D-aspartate (NMDA) receptors by glutamate (Halpainet al.1990). Conversely when phosphorylated at threonine-75 by CDK5, DARPP-32 becomes an inhibitor of protein kinase A (Bibbet al.1999).DARPP-32 is principally expressed in striatal medium spiny neurons and plays a critical role in the regulation of dopaminergic neurotransmission.|
|Preservative & Stabilisers||0.09% Sodium Azide; 0.01% Bovine Serum Albumin; 50% Glycerol|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Darpp-32 (pThr75) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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