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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Darpp-32 (pThr75) Antibody    

Anti-Darpp-32 (pThr75) Antibody

Rabbit Anti-Rat Polyclonal Antibody

  • WB - Anti-Darpp-32 (pThr75) Antibody  ABD11471
    Western blot of rat caudate lysate showing phosphospecific immunolabeling of the ~32 kDa DARPP-32 protein phosphorylated at Thr75
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q6J4I0
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 22913 Da
Additional Information
Purification Antisera to phosphorylated rat DARPP-32 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen Synthetic phosphopeptide corresponding to an amino acid sequence within DARPP-32 which includes phosphorylated Thr75.
Shelf Life 12 months from date of reconstitution.
Gene ID 360616
Other Names Protein phosphatase 1 regulatory subunit 1B, DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, Ppp1r1b
Target/Specificity Rabbit anti-Rat DARPP-32 (pThr75) antibody recognises rat DARPP-32, when phosphorylated at threonine 75. DARPP-32 is a 205 amino acid ~32 kDa member of the protein phosphatase inhibitor 1 family. Originally discovered as a substrate for cAMP-dependent protein kinase which modulates cell signalling in neurons. DARPP-32 is converted into an inhibitor of protein phosphatase-1 when phosphorylated by protein kinase A at threonine 34, as a result of dopaminergic receptor stimulation (Bibbet al.1999). However this phosphorylation is reversed on stimulation of N-methyl-D-aspartate (NMDA) receptors by glutamate (Halpainet al.1990). Conversely when phosphorylated at threonine-75 by CDK5, DARPP-32 becomes an inhibitor of protein kinase A (Bibbet al.1999).DARPP-32 is principally expressed in striatal medium spiny neurons and plays a critical role in the regulation of dopaminergic neurotransmission.
Preservative & Stabilisers 0.09% Sodium Azide; 0.01% Bovine Serum Albumin; 50% Glycerol
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Darpp-32 (pThr75) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD11471
(40 western blots)
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