|Application ||WB, IHC-F, IF|
|Calculated MW||97295 Da|
|Purification||Antisera to phosphorylated rat dynamin were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||Synthetic phosphopeptide corresponding to an amino acid sequence within dynamin which includes phosphorylated Ser774.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Dynamin-1, 188.8.131.52, B-dynamin, D100, Dynamin, brain, Dnm1, Dnm|
|Target/Specificity||Sheep anti-phosphorylated Dynamin antibody recognizes rat dynamin when phosphorylated at serine 774.Dynamin is a phosphoprotein expressed at nerve terminals, which plays an important role in the regulation of synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 (cdk5) has been shown to phosphorylate serine 774 and 778 in vivo. Dephosphorylation of these residues is essential for endocytosis of synaptic vesicles.|
|Preservative & Stabilisers||0.09% Sodium Azide; 0.01% Bovine Serum Albumin; 50% Glycerol|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Dynamin (pSer774) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Morton, A. et al. (2015) Synaptic vesicle exocytosis and increased cytosolic calcium are both necessary but not sufficient for activity-dependent bulk endocytosis.J Neurochem. 134 (3): 405-15. 2. Marland, J.R. et al. (2016) Synaptic Vesicle Recycling Is Unaffected in the Ts65Dn Mouse Model of Down Syndrome.PLoS One. 11 (1): e0147974.
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