|Application ||WB, IHC-F, IF|
|Calculated MW||55966 Da|
|Purification||Antisera to phosphorylated rat tyrosine hydroxylase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||Synthetic phosphopeptide corresponding to an amino acid sequence within Tyrosine Hydroxylase which includes the phosphorylated residue Ser31.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Tyrosine 3-monooxygenase, 184.108.40.206, Tyrosine 3-hydroxylase, TH, Th|
|Target/Specificity||Rabbit anti-phosphorylated tyrosine hydroxylase antibody resognizes tyrosine hydroxylase, when phosphorylated at serine 31.Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the biosynthetic pathway of the catecholamines, dopamine (DA), norepinephrine and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain.Phosphorylation of TH has been shown to occur at several serine residues, including serine 8, 19, 31 and 40.Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson’s disease (Pearsonet al.1979).|
|Preservative & Stabilisers||0.09% Sodium Azide; 0.01% Bovine Serum Albumin; 50% Glycerol|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Tyrosine Hydroxylase (pSer31) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Bevilaqua, L.R.M. et al. (2001) Phosphorylation of Ser19 alters the conformation of tyrosine hydroxylase to increase the rate of phosphorylation of Ser40.J. Biol. Chem. 276: 40411 - 40416. 2. Haycock, J.W. et al. (1990) Phosphorylation of tyrosine hydroxylase in situ at serine 8, 19, 31, and 40.J. Biol. Chem. 265: 11682 - 11691. 3. Lew, J.H. et al. (1999) Increased site-specific phosphorylation of tyrosine hydroxylase accompanies stimulation of enzymatic activity induced by cessation of dopamine neuronal activity.Mol. Pharmacol. 55: 202 - 209.
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