|Application ||WB, IHC-F, IF|
|Calculated MW||58600 Da|
|Purification||Antisera to phosphorylated tyrosine hydroxylase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||Synthetic phosphopeptide corresponding to an amino acid sequence within Tyrosine Hydroxylase which includes the phosphorylated residue Ser 40.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Tyrosine 3-monooxygenase, 188.8.131.52, Tyrosine 3-hydroxylase, TH, TH, TYH|
|Target/Specificity||Rabbit anti-phosphorylated tyrosine hydroxylase antibody recognizes tyrosine hydroxylase, when phosphorylated at serine 40.Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the biosynthetic pathway of the catecholamines dopamine (DA), norepinephrine, and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain.Phosphorylation of TH has been shown to occur at several serine residues. Phosphorylation at serine 40 results in an increase in hydroxylase activity, and phosphorylation at serine 19 is reported to promote phosphorylation of the serine 40 residue (Dunkleyet al.2004). Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson’s disease (Pearsonet al1979).|
|Preservative & Stabilisers||0.09% Sodium Azide; 0.01% Bovine Serum Albumin; 50% Glycerol|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Tyrosine Hydroxylase (pSer40) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Xiao, M.F. et al. (2009) Neural cell adhesion molecule modulates dopaminergic signaling and behavior by regulating dopamine D2 receptor internalization.J Neurosci. 29: 14752-63. 2. Haycock, J.W. et al. (1990) Phosphorylation of tyrosine hydroxylase in situ at serine 8, 19, 31, and 40.J. Biol. Chem. 265: 11682 - 11691. 3. Hoard, J.L. et al. (2008) Cholinergic neurons of mouse intrinsic cardiac ganglia contain noradrenergic enzymes, norepinephrine transporters, and the neurotrophin receptors tropomyosin-related kinase A and p75.Neuroscience. 156: 129-42. 4. Li, S. et al. (2013) The Neural Cell Adhesion Molecule (NCAM) Associates with and Signals through p21-Activated Kinase 1 (Pak1).J Neurosci. 33: 790-803.1. Bevilaqua, L.R.M. et al. (2001) Phosphorylation of Ser19 alters the conformation of tyrosine hydroxylase to increase the rate of phosphorylation of Ser40.J. Biol. Chem. 276: 40411 - 40416.
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